Winogradskyella wandonensis sp. nov., isolated from a tidal flat

A Gram-stain-negative, non-flagellated, non-gliding, aerobic, rod-shaped bacterium, designated WD-2-2^T, was isolated from a tidal flat of Wando, an island of South Korea, and subjected to a polyphasic taxonomic analysis. Strain WD-2-2^T grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain WD-2-2^T belonged to the genus Winogradskyella, clustering coherently with the type strain of Winogradskyella litorisediminis. Strain WD-2-2^T exhibited 16S rRNA gene sequence similarity of 97.4% to W. litorisediminis DPS-8^T and 94.5-96.6% to the type strains of the other species of the genus Winogradskyella. Strain WD-2-2^T contained MK-6 as the predominant menaquinone and iso-C_{15: 1} G, iso-C_{17: 0} 3-OH and iso-C_{15: 0} as the major fatty acids. The major polar lipids detected in strain WD-2-2^T were phosphatidylethanolamine, one unidentified lipid and one unidentified aminolipid. The DNA G+C content was 36.4 mol%, and DNA-DNA relatedness with W. litorisediminis DPS-8^T was 13 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain WD-2- 2^T is separate from recognized species of the genus Winogradskyella. On the basis of the data presented, strain WD-2-2^T is considered to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella wandonensis sp. nov. is proposed. The type strain is WD-2-2^T (=KCTC 32579^T=CECT 8445^T).