Ultrasensitive Cd²⁺ detection based on biomimetic magneto-Au nano-urchin SERS chip fabricated using a 3D printed magnetic mold

Cadmium is a representative carcinogenic heavy metal. Because of the long biological half-life of cadmium, it is critical to prevent and detect cadmium inflow into the body. In this study, we developed the biomimetic magneto-gold nano-urchin (MGNU)-based surface-enhanced Raman scattering (SERS) chip for ultrasensitive detection of cadmium. The MGNU SERS chip was facilely fabricated using three-dimensional (3D) printed magnetic molds. The 3D printed magnetic molds were designed for contributing to (1) making hydrophobic/hydrophilic areas and (2) magnetic SERS enhancement by attracting the MGNUs. To validate the performance of the MGNU SERS chip, we conducted electromagnetic simulations and measurements of SERS efficiencies. Consequently, we detected cadmium ions up to 1.33 pM in distilled water. Moreover, we succeeded to detect cadmium ions in the real environmental samples up to 2.76 pM in the tap water and 14.21 pM in the human blood plasma, respectively. The MGNU SERS chip is a powerful SERS substrate that can be used in various spectrometer-based sensing platforms.