Toward Integrated Multi-omics Analysis for Improving CHO Cell Bioprocessing

Kok Siong Ang; Jongkwang Hong; Meiyappan Lakshmanan; Dong Yup Lee

doi:10.1002/9783527811410.ch7

Toward Integrated Multi-omics Analysis for Improving CHO Cell Bioprocessing

Kok Siong Ang
, Jongkwang Hong
, Meiyappan Lakshmanan
, Dong Yup Lee

Department of Chemical Engineering

Agency for Science, Technology and Research, Singapore

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

3 Scopus citations

Abstract

Chinese hamster ovary (CHO) cells are the most popular mammalian host cells used in the production of human-compatible recombinant drugs because of their capability to perform humanized posttranscriptional modifications such as desired protein folding and N-glycosylation. Through decades of process development and cell line engineering, CHO production titer has now reached 10g/l. As continuing efforts, increasingly various high-throughput omics data have been profiled and analyzed to improve cell culture performance further. Now, the next big challenge is to integrate such heterogeneous omics data systematically. Here, we summarize the current omics techniques from analytics point of view and highlight exemplar multi-omics studies on CHO cell line engineering and bioprocessing.

Original language	English
Title of host publication	Cell Culture Engineering
Subtitle of host publication	Recombinant Protein Production
Publisher	wiley
Pages	163-184
Number of pages	22
ISBN (Electronic)	9783527811410
ISBN (Print)	9783527343348
DOIs	https://doi.org/10.1002/9783527811410.ch7
State	Published - 1 Jan 2019

Keywords

Chinese hamster ovary cells
high-throughput technology
mammalian systems biotechnology
multi-omics data

Access to Document

10.1002/9783527811410.ch7

Cite this

@inbook{e5abfcdd3b0145e7a104bf79583d0203,

title = "Toward Integrated Multi-omics Analysis for Improving CHO Cell Bioprocessing",

abstract = "Chinese hamster ovary (CHO) cells are the most popular mammalian host cells used in the production of human-compatible recombinant drugs because of their capability to perform humanized posttranscriptional modifications such as desired protein folding and N-glycosylation. Through decades of process development and cell line engineering, CHO production titer has now reached 10g/l. As continuing efforts, increasingly various high-throughput omics data have been profiled and analyzed to improve cell culture performance further. Now, the next big challenge is to integrate such heterogeneous omics data systematically. Here, we summarize the current omics techniques from analytics point of view and highlight exemplar multi-omics studies on CHO cell line engineering and bioprocessing.",

keywords = "Chinese hamster ovary cells, high-throughput technology, mammalian systems biotechnology, multi-omics data",

author = "Ang, \{Kok Siong\} and Jongkwang Hong and Meiyappan Lakshmanan and Lee, \{Dong Yup\}",

note = "Publisher Copyright: {\textcopyright} 2020 Wiley-VCH Verlag GmbH \& Co.",

year = "2019",

month = jan,

day = "1",

doi = "10.1002/9783527811410.ch7",

language = "English",

isbn = "9783527343348",

pages = "163--184",

booktitle = "Cell Culture Engineering",

publisher = "wiley",

}

TY - CHAP

T1 - Toward Integrated Multi-omics Analysis for Improving CHO Cell Bioprocessing

AU - Ang, Kok Siong

AU - Hong, Jongkwang

AU - Lakshmanan, Meiyappan

AU - Lee, Dong Yup

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Chinese hamster ovary (CHO) cells are the most popular mammalian host cells used in the production of human-compatible recombinant drugs because of their capability to perform humanized posttranscriptional modifications such as desired protein folding and N-glycosylation. Through decades of process development and cell line engineering, CHO production titer has now reached 10g/l. As continuing efforts, increasingly various high-throughput omics data have been profiled and analyzed to improve cell culture performance further. Now, the next big challenge is to integrate such heterogeneous omics data systematically. Here, we summarize the current omics techniques from analytics point of view and highlight exemplar multi-omics studies on CHO cell line engineering and bioprocessing.

AB - Chinese hamster ovary (CHO) cells are the most popular mammalian host cells used in the production of human-compatible recombinant drugs because of their capability to perform humanized posttranscriptional modifications such as desired protein folding and N-glycosylation. Through decades of process development and cell line engineering, CHO production titer has now reached 10g/l. As continuing efforts, increasingly various high-throughput omics data have been profiled and analyzed to improve cell culture performance further. Now, the next big challenge is to integrate such heterogeneous omics data systematically. Here, we summarize the current omics techniques from analytics point of view and highlight exemplar multi-omics studies on CHO cell line engineering and bioprocessing.

KW - Chinese hamster ovary cells

KW - high-throughput technology

KW - mammalian systems biotechnology

KW - multi-omics data

UR - https://www.scopus.com/pages/publications/105001317425

U2 - 10.1002/9783527811410.ch7

DO - 10.1002/9783527811410.ch7

M3 - Chapter

AN - SCOPUS:105001317425

SN - 9783527343348

SP - 163

EP - 184

BT - Cell Culture Engineering

PB - wiley

ER -

Toward Integrated Multi-omics Analysis for Improving CHO Cell Bioprocessing

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this