The potential of exosomes derived from chronic myelogenous leukaemia cells as a biomarker

Ka Won Kang; Jik Han Jung; Woojune Hur; Jaena Park; Hyunku Shin; Byeonghyeon Choi; Hyesun Jeong; Dae Sik Kim; Eun Sang Yu; Se Ryeon Lee; Hwa Jung Sung; Seok Jin Kim; Chul Won Choi; Hyun Koo Kim; Sunghoi Hong; Ji Ho Park; Yeonho Choi; Yong Park; Byung Soo Kim

doi:10.21873/anticanres.12679

The potential of exosomes derived from chronic myelogenous leukaemia cells as a biomarker

Ka Won Kang
, Jik Han Jung
, Woojune Hur
, Jaena Park
, Hyunku Shin
, Byeonghyeon Choi
, Hyesun Jeong
, Dae Sik Kim
, Eun Sang Yu
, Se Ryeon Lee
, Hwa Jung Sung
, Seok Jin Kim
, Chul Won Choi
, Hyun Koo Kim
, Sunghoi Hong
, Ji Ho Park
, Yeonho Choi
, Yong Park
, Byung Soo Kim

Department of Internal Medicine

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Background/Aim: Exosomes, derived from chronic myelogenous leukaemia (CML) cells, can be used as biomarkers and new targets for the detection of the BCR-ABL transcript. This study aimed to identify these possibilities. Materials and Methods: Human CML cell line-derived exosomes and CML-patients-derived exosomes were isolated with a size-exclusion chromatography column and ExoQuick™ exosome precipitation solution, respectively. Isolated exosomes were analysed by nested PCR to detect the BCR-ABL transcript. Results: Exosomes derived from the two human CML cell lines yielded a 250-bp band. RNA sequence analysis revealed 99% sequence homology with the partial mRNA for the human BCR-ABL chimeric protein. This ~250-bp band was also observed in the exosomes derived from patients with CML. However, only patients at the blast and accelerated phases showed the exosomal BCR-ABL transcript. Conclusion: CML-derived exosomes could act as novel targets for the detection of the BCR-ABL transcript.

Original language	English
Pages (from-to)	3935-3942
Number of pages	8
Journal	Anticancer Research
Volume	38
Issue number	7
DOIs	https://doi.org/10.21873/anticanres.12679
State	Published - Jul 2018

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

BCR-ABL
Biomarker
Chronic myelogenous leukaemia
Exosome

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10.21873/anticanres.12679

Cite this

Kang, K. W., Jung, J. H., Hur, W., Park, J., Shin, H., Choi, B., Jeong, H., Kim, D. S., Yu, E. S., Lee, S. R., Sung, H. J., Kim, S. J., Choi, C. W., Kim, H. K., Hong, S., Park, J. H., Choi, Y., Park, Y., & Kim, B. S. (2018). The potential of exosomes derived from chronic myelogenous leukaemia cells as a biomarker. Anticancer Research, 38(7), 3935-3942. https://doi.org/10.21873/anticanres.12679

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title = "The potential of exosomes derived from chronic myelogenous leukaemia cells as a biomarker",

abstract = "Background/Aim: Exosomes, derived from chronic myelogenous leukaemia (CML) cells, can be used as biomarkers and new targets for the detection of the BCR-ABL transcript. This study aimed to identify these possibilities. Materials and Methods: Human CML cell line-derived exosomes and CML-patients-derived exosomes were isolated with a size-exclusion chromatography column and ExoQuick{\texttrademark} exosome precipitation solution, respectively. Isolated exosomes were analysed by nested PCR to detect the BCR-ABL transcript. Results: Exosomes derived from the two human CML cell lines yielded a 250-bp band. RNA sequence analysis revealed 99\% sequence homology with the partial mRNA for the human BCR-ABL chimeric protein. This \textasciitilde{}250-bp band was also observed in the exosomes derived from patients with CML. However, only patients at the blast and accelerated phases showed the exosomal BCR-ABL transcript. Conclusion: CML-derived exosomes could act as novel targets for the detection of the BCR-ABL transcript.",

keywords = "BCR-ABL, Biomarker, Chronic myelogenous leukaemia, Exosome",

author = "Kang, \{Ka Won\} and Jung, \{Jik Han\} and Woojune Hur and Jaena Park and Hyunku Shin and Byeonghyeon Choi and Hyesun Jeong and Kim, \{Dae Sik\} and Yu, \{Eun Sang\} and Lee, \{Se Ryeon\} and Sung, \{Hwa Jung\} and Kim, \{Seok Jin\} and Choi, \{Chul Won\} and Kim, \{Hyun Koo\} and Sunghoi Hong and Park, \{Ji Ho\} and Yeonho Choi and Yong Park and Kim, \{Byung Soo\}",

year = "2018",

month = jul,

doi = "10.21873/anticanres.12679",

language = "English",

volume = "38",

pages = "3935--3942",

journal = "Anticancer Research",

issn = "0250-7005",

publisher = "International Institute of Anticancer Research",

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TY - JOUR

T1 - The potential of exosomes derived from chronic myelogenous leukaemia cells as a biomarker

AU - Kang, Ka Won

AU - Jung, Jik Han

AU - Hur, Woojune

AU - Park, Jaena

AU - Shin, Hyunku

AU - Choi, Byeonghyeon

AU - Jeong, Hyesun

AU - Kim, Dae Sik

AU - Yu, Eun Sang

AU - Lee, Se Ryeon

AU - Sung, Hwa Jung

AU - Kim, Seok Jin

AU - Choi, Chul Won

AU - Kim, Hyun Koo

AU - Hong, Sunghoi

AU - Park, Ji Ho

AU - Choi, Yeonho

AU - Park, Yong

AU - Kim, Byung Soo

PY - 2018/7

Y1 - 2018/7

N2 - Background/Aim: Exosomes, derived from chronic myelogenous leukaemia (CML) cells, can be used as biomarkers and new targets for the detection of the BCR-ABL transcript. This study aimed to identify these possibilities. Materials and Methods: Human CML cell line-derived exosomes and CML-patients-derived exosomes were isolated with a size-exclusion chromatography column and ExoQuick™ exosome precipitation solution, respectively. Isolated exosomes were analysed by nested PCR to detect the BCR-ABL transcript. Results: Exosomes derived from the two human CML cell lines yielded a 250-bp band. RNA sequence analysis revealed 99% sequence homology with the partial mRNA for the human BCR-ABL chimeric protein. This ~250-bp band was also observed in the exosomes derived from patients with CML. However, only patients at the blast and accelerated phases showed the exosomal BCR-ABL transcript. Conclusion: CML-derived exosomes could act as novel targets for the detection of the BCR-ABL transcript.

AB - Background/Aim: Exosomes, derived from chronic myelogenous leukaemia (CML) cells, can be used as biomarkers and new targets for the detection of the BCR-ABL transcript. This study aimed to identify these possibilities. Materials and Methods: Human CML cell line-derived exosomes and CML-patients-derived exosomes were isolated with a size-exclusion chromatography column and ExoQuick™ exosome precipitation solution, respectively. Isolated exosomes were analysed by nested PCR to detect the BCR-ABL transcript. Results: Exosomes derived from the two human CML cell lines yielded a 250-bp band. RNA sequence analysis revealed 99% sequence homology with the partial mRNA for the human BCR-ABL chimeric protein. This ~250-bp band was also observed in the exosomes derived from patients with CML. However, only patients at the blast and accelerated phases showed the exosomal BCR-ABL transcript. Conclusion: CML-derived exosomes could act as novel targets for the detection of the BCR-ABL transcript.

KW - BCR-ABL

KW - Biomarker

KW - Chronic myelogenous leukaemia

KW - Exosome

UR - https://www.scopus.com/pages/publications/85049772803

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DO - 10.21873/anticanres.12679

M3 - Article

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AN - SCOPUS:85049772803

SN - 0250-7005

VL - 38

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EP - 3942

JO - Anticancer Research

JF - Anticancer Research

IS - 7

ER -

The potential of exosomes derived from chronic myelogenous leukaemia cells as a biomarker

Abstract

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Keywords

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