The novel phospholipase C activator, m-3M3FBS, induces monocytic leukemia cell apoptosis

Youl Nam Lee; Ha Young Lee; Jae Seok Kim; Cheol Park; Hyun Choi Yung; Taehoon G. Lee; Ho Ryu Sung; Jong Young Kwak; Yoe Sik Bae

doi:10.1016/j.canlet.2004.09.017

The novel phospholipase C activator, m-3M3FBS, induces monocytic leukemia cell apoptosis

Youl Nam Lee
, Ha Young Lee
, Jae Seok Kim
, Cheol Park
, Hyun Choi Yung
, Taehoon G. Lee
, Ho Ryu Sung
, Jong Young Kwak
, Yoe Sik Bae

Dong-A University
Dongeui University
Sigmol Incorporation
Pohang University of Science and Technology

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

We investigated the effect of the novel phospholipase C activator, m-3M3FBS, on the apoptosis of leukemic cells. m-3M3FBS inhibited the growth of the leukemic cell lines U937 and THP-1, but not primary monocytes. m-3M3FBS induced the apoptosis of U937 cells, which was accompanied by chromatin condensation and DNA fragmentation. Moreover, m-3M3FBS-induced apoptosis appeared to involve the down-regulation of anti-apoptotic Bcl-2, the up-regulation of pro-apoptotic Bax, the release of cytochrome c, and caspase activation. m-3M3FBS-induced apoptosis of U937 cells was also partly inhibited by BAPTA-AM and EGTA, indicating the involvement of intracellular calcium signaling on the apoptosis in U937 cells. The results of our study suggest that m-3M3FBS can be developed as a novel anti-leukemic agent.

Original language	English
Pages (from-to)	227-235
Number of pages	9
Journal	Cancer Letters
Volume	222
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2004.09.017
State	Published - 26 May 2005
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Apoptosis
Bcl-2
Ca
Caspase
m-3M3FBS
U937

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10.1016/j.canlet.2004.09.017

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title = "The novel phospholipase C activator, m-3M3FBS, induces monocytic leukemia cell apoptosis",

abstract = "We investigated the effect of the novel phospholipase C activator, m-3M3FBS, on the apoptosis of leukemic cells. m-3M3FBS inhibited the growth of the leukemic cell lines U937 and THP-1, but not primary monocytes. m-3M3FBS induced the apoptosis of U937 cells, which was accompanied by chromatin condensation and DNA fragmentation. Moreover, m-3M3FBS-induced apoptosis appeared to involve the down-regulation of anti-apoptotic Bcl-2, the up-regulation of pro-apoptotic Bax, the release of cytochrome c, and caspase activation. m-3M3FBS-induced apoptosis of U937 cells was also partly inhibited by BAPTA-AM and EGTA, indicating the involvement of intracellular calcium signaling on the apoptosis in U937 cells. The results of our study suggest that m-3M3FBS can be developed as a novel anti-leukemic agent.",

keywords = "Apoptosis, Bcl-2, Ca, Caspase, m-3M3FBS, U937",

author = "Lee, \{Youl Nam\} and Lee, \{Ha Young\} and Kim, \{Jae Seok\} and Cheol Park and Yung, \{Hyun Choi\} and Lee, \{Taehoon G.\} and Sung, \{Ho Ryu\} and Kwak, \{Jong Young\} and Bae, \{Yoe Sik\}",

year = "2005",

month = may,

day = "26",

doi = "10.1016/j.canlet.2004.09.017",

language = "English",

volume = "222",

pages = "227--235",

journal = "Cancer Letters",

issn = "0304-3835",

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TY - JOUR

T1 - The novel phospholipase C activator, m-3M3FBS, induces monocytic leukemia cell apoptosis

AU - Lee, Youl Nam

AU - Lee, Ha Young

AU - Kim, Jae Seok

AU - Park, Cheol

AU - Yung, Hyun Choi

AU - Lee, Taehoon G.

AU - Sung, Ho Ryu

AU - Kwak, Jong Young

AU - Bae, Yoe Sik

PY - 2005/5/26

Y1 - 2005/5/26

N2 - We investigated the effect of the novel phospholipase C activator, m-3M3FBS, on the apoptosis of leukemic cells. m-3M3FBS inhibited the growth of the leukemic cell lines U937 and THP-1, but not primary monocytes. m-3M3FBS induced the apoptosis of U937 cells, which was accompanied by chromatin condensation and DNA fragmentation. Moreover, m-3M3FBS-induced apoptosis appeared to involve the down-regulation of anti-apoptotic Bcl-2, the up-regulation of pro-apoptotic Bax, the release of cytochrome c, and caspase activation. m-3M3FBS-induced apoptosis of U937 cells was also partly inhibited by BAPTA-AM and EGTA, indicating the involvement of intracellular calcium signaling on the apoptosis in U937 cells. The results of our study suggest that m-3M3FBS can be developed as a novel anti-leukemic agent.

AB - We investigated the effect of the novel phospholipase C activator, m-3M3FBS, on the apoptosis of leukemic cells. m-3M3FBS inhibited the growth of the leukemic cell lines U937 and THP-1, but not primary monocytes. m-3M3FBS induced the apoptosis of U937 cells, which was accompanied by chromatin condensation and DNA fragmentation. Moreover, m-3M3FBS-induced apoptosis appeared to involve the down-regulation of anti-apoptotic Bcl-2, the up-regulation of pro-apoptotic Bax, the release of cytochrome c, and caspase activation. m-3M3FBS-induced apoptosis of U937 cells was also partly inhibited by BAPTA-AM and EGTA, indicating the involvement of intracellular calcium signaling on the apoptosis in U937 cells. The results of our study suggest that m-3M3FBS can be developed as a novel anti-leukemic agent.

KW - Apoptosis

KW - Bcl-2

KW - Ca

KW - Caspase

KW - m-3M3FBS

KW - U937

UR - https://www.scopus.com/pages/publications/18044381911

U2 - 10.1016/j.canlet.2004.09.017

DO - 10.1016/j.canlet.2004.09.017

M3 - Article

C2 - 15863272

AN - SCOPUS:18044381911

SN - 0304-3835

VL - 222

SP - 227

EP - 235

JO - Cancer Letters

JF - Cancer Letters

IS - 2

ER -

The novel phospholipase C activator, m-3M3FBS, induces monocytic leukemia cell apoptosis

Abstract

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Keywords

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