The novel phospholipase C activator, m-3M3FBS, induces monocytic leukemia cell apoptosis

Youl Nam Lee; Ha Young Lee; Jae Seok Kim; Cheol Park; Hyun Choi Yung; Taehoon G. Lee; Ho Ryu Sung; Jong Young Kwak; Yoe Sik Bae

doi:10.1016/j.canlet.2004.09.017

The novel phospholipase C activator, m-3M3FBS, induces monocytic leukemia cell apoptosis

Youl Nam Lee, Ha Young Lee, Jae Seok Kim, Cheol Park, Hyun Choi Yung, Taehoon G. Lee, Ho Ryu Sung, Jong Young Kwak, Yoe Sik Bae

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

We investigated the effect of the novel phospholipase C activator, m-3M3FBS, on the apoptosis of leukemic cells. m-3M3FBS inhibited the growth of the leukemic cell lines U937 and THP-1, but not primary monocytes. m-3M3FBS induced the apoptosis of U937 cells, which was accompanied by chromatin condensation and DNA fragmentation. Moreover, m-3M3FBS-induced apoptosis appeared to involve the down-regulation of anti-apoptotic Bcl-2, the up-regulation of pro-apoptotic Bax, the release of cytochrome c, and caspase activation. m-3M3FBS-induced apoptosis of U937 cells was also partly inhibited by BAPTA-AM and EGTA, indicating the involvement of intracellular calcium signaling on the apoptosis in U937 cells. The results of our study suggest that m-3M3FBS can be developed as a novel anti-leukemic agent.

Original language	English
Pages (from-to)	227-235
Number of pages	9
Journal	Cancer Letters
Volume	222
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2004.09.017
State	Published - 26 May 2005
Externally published	Yes

Keywords

Apoptosis
Bcl-2
Ca
Caspase
m-3M3FBS
U937

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.canlet.2004.09.017

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title = "The novel phospholipase C activator, m-3M3FBS, induces monocytic leukemia cell apoptosis",

abstract = "We investigated the effect of the novel phospholipase C activator, m-3M3FBS, on the apoptosis of leukemic cells. m-3M3FBS inhibited the growth of the leukemic cell lines U937 and THP-1, but not primary monocytes. m-3M3FBS induced the apoptosis of U937 cells, which was accompanied by chromatin condensation and DNA fragmentation. Moreover, m-3M3FBS-induced apoptosis appeared to involve the down-regulation of anti-apoptotic Bcl-2, the up-regulation of pro-apoptotic Bax, the release of cytochrome c, and caspase activation. m-3M3FBS-induced apoptosis of U937 cells was also partly inhibited by BAPTA-AM and EGTA, indicating the involvement of intracellular calcium signaling on the apoptosis in U937 cells. The results of our study suggest that m-3M3FBS can be developed as a novel anti-leukemic agent.",

keywords = "Apoptosis, Bcl-2, Ca, Caspase, m-3M3FBS, U937",

author = "Lee, \{Youl Nam\} and Lee, \{Ha Young\} and Kim, \{Jae Seok\} and Cheol Park and Yung, \{Hyun Choi\} and Lee, \{Taehoon G.\} and Sung, \{Ho Ryu\} and Kwak, \{Jong Young\} and Bae, \{Yoe Sik\}",

year = "2005",

month = may,

day = "26",

doi = "10.1016/j.canlet.2004.09.017",

language = "English",

volume = "222",

pages = "227--235",

journal = "Cancer Letters",

issn = "0304-3835",

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TY - JOUR

T1 - The novel phospholipase C activator, m-3M3FBS, induces monocytic leukemia cell apoptosis

AU - Lee, Youl Nam

AU - Lee, Ha Young

AU - Kim, Jae Seok

AU - Park, Cheol

AU - Yung, Hyun Choi

AU - Lee, Taehoon G.

AU - Sung, Ho Ryu

AU - Kwak, Jong Young

AU - Bae, Yoe Sik

PY - 2005/5/26

Y1 - 2005/5/26

N2 - We investigated the effect of the novel phospholipase C activator, m-3M3FBS, on the apoptosis of leukemic cells. m-3M3FBS inhibited the growth of the leukemic cell lines U937 and THP-1, but not primary monocytes. m-3M3FBS induced the apoptosis of U937 cells, which was accompanied by chromatin condensation and DNA fragmentation. Moreover, m-3M3FBS-induced apoptosis appeared to involve the down-regulation of anti-apoptotic Bcl-2, the up-regulation of pro-apoptotic Bax, the release of cytochrome c, and caspase activation. m-3M3FBS-induced apoptosis of U937 cells was also partly inhibited by BAPTA-AM and EGTA, indicating the involvement of intracellular calcium signaling on the apoptosis in U937 cells. The results of our study suggest that m-3M3FBS can be developed as a novel anti-leukemic agent.

AB - We investigated the effect of the novel phospholipase C activator, m-3M3FBS, on the apoptosis of leukemic cells. m-3M3FBS inhibited the growth of the leukemic cell lines U937 and THP-1, but not primary monocytes. m-3M3FBS induced the apoptosis of U937 cells, which was accompanied by chromatin condensation and DNA fragmentation. Moreover, m-3M3FBS-induced apoptosis appeared to involve the down-regulation of anti-apoptotic Bcl-2, the up-regulation of pro-apoptotic Bax, the release of cytochrome c, and caspase activation. m-3M3FBS-induced apoptosis of U937 cells was also partly inhibited by BAPTA-AM and EGTA, indicating the involvement of intracellular calcium signaling on the apoptosis in U937 cells. The results of our study suggest that m-3M3FBS can be developed as a novel anti-leukemic agent.

KW - Apoptosis

KW - Bcl-2

KW - Ca

KW - Caspase

KW - m-3M3FBS

KW - U937

UR - https://www.scopus.com/pages/publications/18044381911

U2 - 10.1016/j.canlet.2004.09.017

DO - 10.1016/j.canlet.2004.09.017

M3 - Article

C2 - 15863272

AN - SCOPUS:18044381911

SN - 0304-3835

VL - 222

SP - 227

EP - 235

JO - Cancer Letters

JF - Cancer Letters

IS - 2

ER -

The novel phospholipase C activator, m-3M3FBS, induces monocytic leukemia cell apoptosis

Abstract

Keywords

UN SDGs

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