The DNA hybridization assay using single-walled carbon nanotubes as ultrasensitive, long-term optical labels

Eung Soo Hwang; Chengfan Cao; Sanghyun Hong; Hye Jin Jung; Chang Yong Cha; Jae Boong Choi; Young Jin Kim; Seunghyun Baik

doi:10.1088/0957-4484/17/14/016

The DNA hybridization assay using single-walled carbon nanotubes as ultrasensitive, long-term optical labels

Eung Soo Hwang
, Chengfan Cao
, Sanghyun Hong
, Hye Jin Jung
, Chang Yong Cha
, Jae Boong Choi
, Young Jin Kim
, Seunghyun Baik

Department of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Single walled carbon nanotubes (SWNTs) exhibit strong Raman signals as well as fluorescence emissions in the near infrared region. Such signals do not blink or photobleach under prolonged excitation, which is an advantage in optical nano-biomarker applications. In this paper, we present single-stranded DNA conjugated SWNT probes to locate a particular sequence of DNA within a complex genome. Chromosomal DNAs of human fibroblasts and Escherichia coli are used as a target and a control, respectively. Southern blotting, which uses photostable Raman signals of nanotubes instead of fluorescent dyes, demonstrates excellent sensitivity and specificity of the probes. The results show that SWNTs may be used as generic nano-biomarkers for the precise detection of specific kinds of genes.

Original language	English
Article number	016
Pages (from-to)	3442-3445
Number of pages	4
Journal	Nanotechnology
Volume	17
Issue number	14
DOIs	https://doi.org/10.1088/0957-4484/17/14/016
State	Published - 28 Jul 2006

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10.1088/0957-4484/17/14/016

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title = "The DNA hybridization assay using single-walled carbon nanotubes as ultrasensitive, long-term optical labels",

abstract = "Single walled carbon nanotubes (SWNTs) exhibit strong Raman signals as well as fluorescence emissions in the near infrared region. Such signals do not blink or photobleach under prolonged excitation, which is an advantage in optical nano-biomarker applications. In this paper, we present single-stranded DNA conjugated SWNT probes to locate a particular sequence of DNA within a complex genome. Chromosomal DNAs of human fibroblasts and Escherichia coli are used as a target and a control, respectively. Southern blotting, which uses photostable Raman signals of nanotubes instead of fluorescent dyes, demonstrates excellent sensitivity and specificity of the probes. The results show that SWNTs may be used as generic nano-biomarkers for the precise detection of specific kinds of genes.",

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T1 - The DNA hybridization assay using single-walled carbon nanotubes as ultrasensitive, long-term optical labels

AU - Hwang, Eung Soo

AU - Cao, Chengfan

AU - Hong, Sanghyun

AU - Jung, Hye Jin

AU - Cha, Chang Yong

AU - Choi, Jae Boong

AU - Kim, Young Jin

AU - Baik, Seunghyun

PY - 2006/7/28

Y1 - 2006/7/28

N2 - Single walled carbon nanotubes (SWNTs) exhibit strong Raman signals as well as fluorescence emissions in the near infrared region. Such signals do not blink or photobleach under prolonged excitation, which is an advantage in optical nano-biomarker applications. In this paper, we present single-stranded DNA conjugated SWNT probes to locate a particular sequence of DNA within a complex genome. Chromosomal DNAs of human fibroblasts and Escherichia coli are used as a target and a control, respectively. Southern blotting, which uses photostable Raman signals of nanotubes instead of fluorescent dyes, demonstrates excellent sensitivity and specificity of the probes. The results show that SWNTs may be used as generic nano-biomarkers for the precise detection of specific kinds of genes.

AB - Single walled carbon nanotubes (SWNTs) exhibit strong Raman signals as well as fluorescence emissions in the near infrared region. Such signals do not blink or photobleach under prolonged excitation, which is an advantage in optical nano-biomarker applications. In this paper, we present single-stranded DNA conjugated SWNT probes to locate a particular sequence of DNA within a complex genome. Chromosomal DNAs of human fibroblasts and Escherichia coli are used as a target and a control, respectively. Southern blotting, which uses photostable Raman signals of nanotubes instead of fluorescent dyes, demonstrates excellent sensitivity and specificity of the probes. The results show that SWNTs may be used as generic nano-biomarkers for the precise detection of specific kinds of genes.

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The DNA hybridization assay using single-walled carbon nanotubes as ultrasensitive, long-term optical labels

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