Thalassobacillus hwangdonensis sp. nov., isolated from a tidal flat sediment

A Gram-staining-positive, endospore-forming, motile, rod-shaped bacterium, strain AD-1^T, was isolated from a tidal flat sediment of the coast of Hwangdo on the Yellow Sea, Korea. Strain AD-1^T grew optimally at pH 7.0-7.5 and 40°C and in the presence of 5-10% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain AD-1^T was most closely related to Thalassobacillus devorans G-19.1^T (98.0% sequence similarity) and Thalassobacillus cyri HS286^T (97.8%). The cell-wall peptidoglycan was based on mesodiaminopimelic acid and MK-7 was the predominant menaquinone. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and two unidentified lipids. The major fatty acids (>10% of total fatty acids) were iso-C_{15 : 0}, iso-C_{17 : 0} and anteiso-C_{15 : 0}. The DNA G+C content of strain AD-1^T was 45.2 mol%. It appears reasonable to classify strain AD-1^T as a member of the genus Thalassobacillus. There were differences in fatty acid profiles and phenotypic and genetic characteristics between strain AD-1^T and the type strains of the two Thalassobacillus species. On the basis of the data presented, strain AD-1^T represents a novel species within the genus Thalassobacillus, for which the name Thalassobacillus hwangdonensis sp. nov. is proposed. The type strain is AD-1^T (=KCTC 13254^T =CCUG 56607^T).