Terrabacter terrigena sp. nov., isolated from soil

A Gram-positive-staining, non-motile and rod-shaped bacterium, strain ON10^T, was isolated from soil around a wastewater treatment plant in Korea and its taxonomic position was investigated by using a polyphasic approach. Strain ON10^T grew optimally at pH 6.5-7.0 and 30 °C in the presence of 0.5% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ON10^T clustered with the clade comprising Terrabacter species, with which it exhibited 16S rRNA gene sequence similarity values of 98.4-98.8 %. The cell-wall peptidoglycan type was based on LL-diaminopimelic acid and the cell-wall sugars were glucose, mannose, arabinose and xylose. The predominant menaquinone was MK-8(H₄). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, an unidentified phospholipid and an unidentified lipid. The major fatty acids were iso-C_15:0 and iso- C_14:0. The DNA G+C content was 71.6 mol%. Strain ON10^T exhibited DNA-DNA relatedness levels of 17-28% to the type strains of Terrabacter species and could also be differentiated from these species by differences in phenotypic characteristics. On the basis of the data obtained, strain ON10^T was considered to represent a novel species of the genus Terrabacter, for which the name Terrabacter terrigena sp. nov. is proposed. The type strain is ON10^T (=KCTC 19602^T=CCUG 57508^T).