Synthetic biology for corynebacterium glutamicum: An industrial host for white biotechnology

Synthetic biology is a redefined biological discipline based on engineering concepts with computer-aided tools and has enabled construction of efficient biological systems under precise control by gene expression. Corynebacterium glutamicum is a Gram-positive, facultative anaerobic industrial host (GRAS: generally recognized as safe) that is used for large-scale industrial production of the flavor enhancer L-glutamate and the food additive L-lysine This chapter describes development of synthetic biology platform for Corynebacterium glutamicum toward microbial cell factories for white biotechnology. Promoter is one of the crucial DNA parts essential for gene transcription. Transcriptional regulator regulates the level of gene expression by altering transcription rates in a local genetic circuit or in a global network by different class of regulators. Due to the industrial importance of Corynebacterium glutamicum as an amino acid host, genetic biosensors have been developed using native transcriptional regulators that recognize the amino acids.