Synthesis of 7′-[123I]iodo-D-luciferin for in vivo studies of firefly luciferase gene expression

Sang Yoon Lee; Yearn Seong Choe; Kyung Han Lee; Jeewoo Lee; Yong Choi; Byung Tae Kim

doi:10.1016/j.bmcl.2003.12.066

Synthesis of 7′-[¹²³I]iodo-D-luciferin for in vivo studies of firefly luciferase gene expression

Sang Yoon Lee, Yearn Seong Choe, Kyung Han Lee, Jeewoo Lee, Yong Choi, Byung Tae Kim

Department of Nuclear Medicine

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

D-(-)-2-(6′-Hydroxy-7′-[¹²³I]iodobenzothiazolyl)- Δ²-thiazoline-4-caroxylic acid (7′-[¹²³I]iodo- D-luciferin) was synthesized as a novel reporter probe for in vivo studies of firefly luciferase gene expression. 7′-Iodo-D-luciferin, a nonradioactive standard, was synthesized and showed the binding property (K_M=4.28 μM) similar to that of D-luciferin (2.53 μM) for firefly luciferase in luminescence assay.

Original language	English
Pages (from-to)	1161-1163
Number of pages	3
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	14
Issue number	5
DOIs	https://doi.org/10.1016/j.bmcl.2003.12.066
State	Published - 8 Mar 2004

Keywords

7′-[I]iodo-D- luciferin
Firefly luciferase
Gene expression
Luminescence assay

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10.1016/j.bmcl.2003.12.066

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@article{dce250e1137e494291440a8ff795179b,

title = "Synthesis of 7′-[123I]iodo-D-luciferin for in vivo studies of firefly luciferase gene expression",

abstract = "D-(-)-2-(6′-Hydroxy-7′-[123I]iodobenzothiazolyl)- Δ2-thiazoline-4-caroxylic acid (7′-[123I]iodo- D-luciferin) was synthesized as a novel reporter probe for in vivo studies of firefly luciferase gene expression. 7′-Iodo-D-luciferin, a nonradioactive standard, was synthesized and showed the binding property (KM=4.28 μM) similar to that of D-luciferin (2.53 μM) for firefly luciferase in luminescence assay.",

keywords = "7′-[I]iodo-D- luciferin, Firefly luciferase, Gene expression, Luminescence assay",

author = "Lee, \{Sang Yoon\} and Choe, \{Yearn Seong\} and Lee, \{Kyung Han\} and Jeewoo Lee and Yong Choi and Kim, \{Byung Tae\}",

year = "2004",

month = mar,

day = "8",

doi = "10.1016/j.bmcl.2003.12.066",

language = "English",

volume = "14",

pages = "1161--1163",

journal = "Bioorganic and Medicinal Chemistry Letters",

issn = "0960-894X",

publisher = "Elsevier Ltd",

number = "5",

}

TY - JOUR

T1 - Synthesis of 7′-[123I]iodo-D-luciferin for in vivo studies of firefly luciferase gene expression

AU - Lee, Sang Yoon

AU - Choe, Yearn Seong

AU - Lee, Kyung Han

AU - Lee, Jeewoo

AU - Choi, Yong

AU - Kim, Byung Tae

PY - 2004/3/8

Y1 - 2004/3/8

N2 - D-(-)-2-(6′-Hydroxy-7′-[123I]iodobenzothiazolyl)- Δ2-thiazoline-4-caroxylic acid (7′-[123I]iodo- D-luciferin) was synthesized as a novel reporter probe for in vivo studies of firefly luciferase gene expression. 7′-Iodo-D-luciferin, a nonradioactive standard, was synthesized and showed the binding property (KM=4.28 μM) similar to that of D-luciferin (2.53 μM) for firefly luciferase in luminescence assay.

AB - D-(-)-2-(6′-Hydroxy-7′-[123I]iodobenzothiazolyl)- Δ2-thiazoline-4-caroxylic acid (7′-[123I]iodo- D-luciferin) was synthesized as a novel reporter probe for in vivo studies of firefly luciferase gene expression. 7′-Iodo-D-luciferin, a nonradioactive standard, was synthesized and showed the binding property (KM=4.28 μM) similar to that of D-luciferin (2.53 μM) for firefly luciferase in luminescence assay.

KW - 7′-[I]iodo-D- luciferin

KW - Firefly luciferase

KW - Gene expression

KW - Luminescence assay

UR - https://www.scopus.com/pages/publications/1242316924

U2 - 10.1016/j.bmcl.2003.12.066

DO - 10.1016/j.bmcl.2003.12.066

M3 - Article

C2 - 14980656

AN - SCOPUS:1242316924

SN - 0960-894X

VL - 14

SP - 1161

EP - 1163

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 5

ER -

Synthesis of 7′-[¹²³I]iodo-D-luciferin for in vivo studies of firefly luciferase gene expression

Abstract

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