Synthesis of 7′-[123I]iodo-D-luciferin for in vivo studies of firefly luciferase gene expression

Sang Yoon Lee; Yearn Seong Choe; Kyung Han Lee; Jeewoo Lee; Yong Choi; Byung Tae Kim

doi:10.1016/j.bmcl.2003.12.066

Synthesis of 7′-[¹²³I]iodo-D-luciferin for in vivo studies of firefly luciferase gene expression

Sang Yoon Lee
, Yearn Seong Choe
, Kyung Han Lee
, Jeewoo Lee
, Yong Choi
, Byung Tae Kim

Department of Nuclear Medicine

Sungkyunkwan University
Seoul National University

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

D-(-)-2-(6′-Hydroxy-7′-[¹²³I]iodobenzothiazolyl)- Δ²-thiazoline-4-caroxylic acid (7′-[¹²³I]iodo- D-luciferin) was synthesized as a novel reporter probe for in vivo studies of firefly luciferase gene expression. 7′-Iodo-D-luciferin, a nonradioactive standard, was synthesized and showed the binding property (K_M=4.28 μM) similar to that of D-luciferin (2.53 μM) for firefly luciferase in luminescence assay.

Original language	English
Pages (from-to)	1161-1163
Number of pages	3
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	14
Issue number	5
DOIs	https://doi.org/10.1016/j.bmcl.2003.12.066
State	Published - 8 Mar 2004

Keywords

7′-[I]iodo-D- luciferin
Firefly luciferase
Gene expression
Luminescence assay

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10.1016/j.bmcl.2003.12.066

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@article{dce250e1137e494291440a8ff795179b,

title = "Synthesis of 7′-[123I]iodo-D-luciferin for in vivo studies of firefly luciferase gene expression",

abstract = "D-(-)-2-(6′-Hydroxy-7′-[123I]iodobenzothiazolyl)- Δ2-thiazoline-4-caroxylic acid (7′-[123I]iodo- D-luciferin) was synthesized as a novel reporter probe for in vivo studies of firefly luciferase gene expression. 7′-Iodo-D-luciferin, a nonradioactive standard, was synthesized and showed the binding property (KM=4.28 μM) similar to that of D-luciferin (2.53 μM) for firefly luciferase in luminescence assay.",

keywords = "7′-[I]iodo-D- luciferin, Firefly luciferase, Gene expression, Luminescence assay",

author = "Lee, \{Sang Yoon\} and Choe, \{Yearn Seong\} and Lee, \{Kyung Han\} and Jeewoo Lee and Yong Choi and Kim, \{Byung Tae\}",

year = "2004",

month = mar,

day = "8",

doi = "10.1016/j.bmcl.2003.12.066",

language = "English",

volume = "14",

pages = "1161--1163",

journal = "Bioorganic and Medicinal Chemistry Letters",

issn = "0960-894X",

publisher = "Elsevier Ltd",

number = "5",

}

TY - JOUR

T1 - Synthesis of 7′-[123I]iodo-D-luciferin for in vivo studies of firefly luciferase gene expression

AU - Lee, Sang Yoon

AU - Choe, Yearn Seong

AU - Lee, Kyung Han

AU - Lee, Jeewoo

AU - Choi, Yong

AU - Kim, Byung Tae

PY - 2004/3/8

Y1 - 2004/3/8

N2 - D-(-)-2-(6′-Hydroxy-7′-[123I]iodobenzothiazolyl)- Δ2-thiazoline-4-caroxylic acid (7′-[123I]iodo- D-luciferin) was synthesized as a novel reporter probe for in vivo studies of firefly luciferase gene expression. 7′-Iodo-D-luciferin, a nonradioactive standard, was synthesized and showed the binding property (KM=4.28 μM) similar to that of D-luciferin (2.53 μM) for firefly luciferase in luminescence assay.

AB - D-(-)-2-(6′-Hydroxy-7′-[123I]iodobenzothiazolyl)- Δ2-thiazoline-4-caroxylic acid (7′-[123I]iodo- D-luciferin) was synthesized as a novel reporter probe for in vivo studies of firefly luciferase gene expression. 7′-Iodo-D-luciferin, a nonradioactive standard, was synthesized and showed the binding property (KM=4.28 μM) similar to that of D-luciferin (2.53 μM) for firefly luciferase in luminescence assay.

KW - 7′-[I]iodo-D- luciferin

KW - Firefly luciferase

KW - Gene expression

KW - Luminescence assay

UR - https://www.scopus.com/pages/publications/1242316924

U2 - 10.1016/j.bmcl.2003.12.066

DO - 10.1016/j.bmcl.2003.12.066

M3 - Article

C2 - 14980656

AN - SCOPUS:1242316924

SN - 0960-894X

VL - 14

SP - 1161

EP - 1163

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 5

ER -

Synthesis of 7′-[¹²³I]iodo-D-luciferin for in vivo studies of firefly luciferase gene expression

Abstract

Keywords

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