Sungkyunkwania multivorans gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from seawater from a seaweed farm

A Gram-staining-negative, non-flagellated, non-gliding and rod-shaped bacterial strain, designated PDB-16^T, was isolated from seawater from a seaweed farm on the South Sea in Korea, and its taxonomic position was investigated using a polyphasic approach. Strain PDB-16^T grew optimally at 30 °C, at pH 7.0-7.5 and in the presence of 2% (w/v) NaCl. A neighbour- joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain PDB-16^T formed an independent lineage within the evolutionary radiation encompassed by the family Flavobacteriaceae. Strain PDB-16^T contained MK-6 as the predominant menaquinone and iso- C_15:0, iso-C_15:1 G and iso-C_17:0 3-OH as the major fatty acids. The major polar lipids detected in strain PDB-16^T were phosphatidylethanolamine and two unidentified lipids. The DNA G + C content of strain PDB-16^T was 42.1 mol%. Strain PDB-16^T exhibited very low 16S rRNA gene sequence similarities of less than 89.7% to the type strains of any bacterial species with validly published names and less than 90.1 % to uncultured bacteria clones. The 16S rRNA gene sequence similarity values and the differences in phenotypic properties between strain PDB-16^T and some phylogenetically related genera were sufficient to support the proposal that strain PDB- 16^(t) should be distinguished from previously known genera of the family Flavobacteriaceae. On the basis of the data presented, strain PDB-16^T is considered to represent a new genus and novel species, for which the name Sungkyunkwania multivorans gen. nov., sp. nov. is proposed. The type strain of Sungkyunkwania multivorans is PDB-16^T (=KCTC 32138^T=CCUG 62952^T).