Structure of PAP-IgM FcK fusion protein with J-chain expressed in transgenic plant

Yangjoo Kang; Deuk Su Kim; Kibum Kim; Soon Chul Myung; Yoo Jin Oh; Sungsu Park; Peter Hinterdorfer; Kisung Ko

doi:10.2478/ebtj-2023-0006

Structure of PAP-IgM FcK fusion protein with J-chain expressed in transgenic plant

Yangjoo Kang
, Deuk Su Kim
, Kibum Kim
, Soon Chul Myung
, Yoo Jin Oh
, Sungsu Park
, Peter Hinterdorfer
, Kisung Ko

Department of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Transgenic plants expressing immunoglobulin (Ig) M Fc-fused Prostate acid phosphatase (PAP) antigenic proteins (PAP-IgM FcK) and J-chain proteins were generated by Agrobacterium-mediated transformation. The Fc region was tagged with the ER retention motif (KDEL) to make PAP-IgM FcK. Two transgenic plants were crossed together to generate F1 expressing both PAP-IgM FcK and J-chain proteins (PAP-IgM FcK × J-chain). PCR and RT-PCR analyses confirmed the transgene insertion and mRNA transcription of PAP-IgM FcK and J-chain in leaf tissue of PAP-IgM FcK × J-chain F1 plant. Western blot confirmed the expression of PAP-IgM FcK × J-chain protein. Size exclusion (SEC)-high performance liquid chromatography (HPLC) and Bio-transmission electron microscope (TEM) analyses were performed to show the size and shape of the PAP- IgM FcK × J-chain fusion proteins. These results suggest that PAP-IgM FcK with J-chain can be produced in plant expression system with plant crossing.

Original language	English
Pages (from-to)	87-95
Number of pages	9
Journal	Eurobiotech Journal
Volume	7
Issue number	1
DOIs	https://doi.org/10.2478/ebtj-2023-0006
State	Published - 1 Jan 2023

Keywords

Immunoglobulin
Plant Biotechnology
Protein Engineering
Transgene

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10.2478/ebtj-2023-0006

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title = "Structure of PAP-IgM FcK fusion protein with J-chain expressed in transgenic plant",

abstract = "Transgenic plants expressing immunoglobulin (Ig) M Fc-fused Prostate acid phosphatase (PAP) antigenic proteins (PAP-IgM FcK) and J-chain proteins were generated by Agrobacterium-mediated transformation. The Fc region was tagged with the ER retention motif (KDEL) to make PAP-IgM FcK. Two transgenic plants were crossed together to generate F1 expressing both PAP-IgM FcK and J-chain proteins (PAP-IgM FcK × J-chain). PCR and RT-PCR analyses confirmed the transgene insertion and mRNA transcription of PAP-IgM FcK and J-chain in leaf tissue of PAP-IgM FcK × J-chain F1 plant. Western blot confirmed the expression of PAP-IgM FcK × J-chain protein. Size exclusion (SEC)-high performance liquid chromatography (HPLC) and Bio-transmission electron microscope (TEM) analyses were performed to show the size and shape of the PAP- IgM FcK × J-chain fusion proteins. These results suggest that PAP-IgM FcK with J-chain can be produced in plant expression system with plant crossing.",

keywords = "Immunoglobulin, Plant Biotechnology, Protein Engineering, Transgene",

author = "Yangjoo Kang and Kim, \{Deuk Su\} and Kibum Kim and Myung, \{Soon Chul\} and Oh, \{Yoo Jin\} and Sungsu Park and Peter Hinterdorfer and Kisung Ko",

note = "Publisher Copyright: {\textcopyright} 2023 Yangjoo Kang et al., published by Sciendo.",

year = "2023",

month = jan,

day = "1",

doi = "10.2478/ebtj-2023-0006",

language = "English",

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T1 - Structure of PAP-IgM FcK fusion protein with J-chain expressed in transgenic plant

AU - Kang, Yangjoo

AU - Kim, Deuk Su

AU - Kim, Kibum

AU - Myung, Soon Chul

AU - Oh, Yoo Jin

AU - Park, Sungsu

AU - Hinterdorfer, Peter

AU - Ko, Kisung

PY - 2023/1/1

Y1 - 2023/1/1

N2 - Transgenic plants expressing immunoglobulin (Ig) M Fc-fused Prostate acid phosphatase (PAP) antigenic proteins (PAP-IgM FcK) and J-chain proteins were generated by Agrobacterium-mediated transformation. The Fc region was tagged with the ER retention motif (KDEL) to make PAP-IgM FcK. Two transgenic plants were crossed together to generate F1 expressing both PAP-IgM FcK and J-chain proteins (PAP-IgM FcK × J-chain). PCR and RT-PCR analyses confirmed the transgene insertion and mRNA transcription of PAP-IgM FcK and J-chain in leaf tissue of PAP-IgM FcK × J-chain F1 plant. Western blot confirmed the expression of PAP-IgM FcK × J-chain protein. Size exclusion (SEC)-high performance liquid chromatography (HPLC) and Bio-transmission electron microscope (TEM) analyses were performed to show the size and shape of the PAP- IgM FcK × J-chain fusion proteins. These results suggest that PAP-IgM FcK with J-chain can be produced in plant expression system with plant crossing.

AB - Transgenic plants expressing immunoglobulin (Ig) M Fc-fused Prostate acid phosphatase (PAP) antigenic proteins (PAP-IgM FcK) and J-chain proteins were generated by Agrobacterium-mediated transformation. The Fc region was tagged with the ER retention motif (KDEL) to make PAP-IgM FcK. Two transgenic plants were crossed together to generate F1 expressing both PAP-IgM FcK and J-chain proteins (PAP-IgM FcK × J-chain). PCR and RT-PCR analyses confirmed the transgene insertion and mRNA transcription of PAP-IgM FcK and J-chain in leaf tissue of PAP-IgM FcK × J-chain F1 plant. Western blot confirmed the expression of PAP-IgM FcK × J-chain protein. Size exclusion (SEC)-high performance liquid chromatography (HPLC) and Bio-transmission electron microscope (TEM) analyses were performed to show the size and shape of the PAP- IgM FcK × J-chain fusion proteins. These results suggest that PAP-IgM FcK with J-chain can be produced in plant expression system with plant crossing.

KW - Immunoglobulin

KW - Plant Biotechnology

KW - Protein Engineering

KW - Transgene

UR - https://www.scopus.com/pages/publications/85147305437

U2 - 10.2478/ebtj-2023-0006

DO - 10.2478/ebtj-2023-0006

M3 - Article

AN - SCOPUS:85147305437

SN - 2564-615X

VL - 7

SP - 87

EP - 95

JO - Eurobiotech Journal

JF - Eurobiotech Journal

IS - 1

ER -

Structure of PAP-IgM FcK fusion protein with J-chain expressed in transgenic plant

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