Structure of a GRK5-Calmodulin Complex Reveals Molecular Mechanism of GRK Activation and Substrate Targeting

Konstantin E. Komolov; Sarah M. Sulon; Anshul Bhardwaj; Siri C. van Keulen; Nguyen Minh Duc; Daniela K. Laurinavichyute; Hua Jane Lou; Benjamin E. Turk; Ka Young Chung; Ron O. Dror; Jeffrey L. Benovic

doi:10.1016/j.molcel.2020.11.026

Structure of a GRK5-Calmodulin Complex Reveals Molecular Mechanism of GRK Activation and Substrate Targeting

Konstantin E. Komolov
, Sarah M. Sulon
, Anshul Bhardwaj
, Siri C. van Keulen
, Nguyen Minh Duc
, Daniela K. Laurinavichyute
, Hua Jane Lou
, Benjamin E. Turk
, Ka Young Chung
, Ron O. Dror
, Jeffrey L. Benovic

Department of Pharmacy

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Komolov et al. use structural, computational, and functional approaches to characterize the mechanism of calmodulin-mediated activation of the G protein-coupled receptor kinase GRK5. Additional findings reveal that GRK5 activation and localization cooperate under control of calmodulin for selective targeting of substrates at the plasma membrane and cytoplasm.

Original language	English
Pages (from-to)	323-339.e11
Journal	Molecular Cell
Volume	81
Issue number	2
DOIs	https://doi.org/10.1016/j.molcel.2020.11.026
State	Published - 21 Jan 2021

Keywords

G protein-coupled receptor
X-ray crystallography
calcium
hydrogen/deuterium exchange mass spectrometry
molecular dynamics
phosphorylation
protein kinase

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.molcel.2020.11.026

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title = "Structure of a GRK5-Calmodulin Complex Reveals Molecular Mechanism of GRK Activation and Substrate Targeting",

abstract = "Komolov et al. use structural, computational, and functional approaches to characterize the mechanism of calmodulin-mediated activation of the G protein-coupled receptor kinase GRK5. Additional findings reveal that GRK5 activation and localization cooperate under control of calmodulin for selective targeting of substrates at the plasma membrane and cytoplasm.",

keywords = "G protein-coupled receptor, X-ray crystallography, calcium, hydrogen/deuterium exchange mass spectrometry, molecular dynamics, phosphorylation, protein kinase",

author = "Komolov, \{Konstantin E.\} and Sulon, \{Sarah M.\} and Anshul Bhardwaj and \{van Keulen\}, \{Siri C.\} and Duc, \{Nguyen Minh\} and Laurinavichyute, \{Daniela K.\} and Lou, \{Hua Jane\} and Turk, \{Benjamin E.\} and Chung, \{Ka Young\} and Dror, \{Ron O.\} and Benovic, \{Jeffrey L.\}",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",

year = "2021",

month = jan,

day = "21",

doi = "10.1016/j.molcel.2020.11.026",

language = "English",

volume = "81",

pages = "323--339.e11",

journal = "Molecular Cell",

issn = "1097-2765",

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T1 - Structure of a GRK5-Calmodulin Complex Reveals Molecular Mechanism of GRK Activation and Substrate Targeting

AU - Komolov, Konstantin E.

AU - Sulon, Sarah M.

AU - Bhardwaj, Anshul

AU - van Keulen, Siri C.

AU - Duc, Nguyen Minh

AU - Laurinavichyute, Daniela K.

AU - Lou, Hua Jane

AU - Turk, Benjamin E.

AU - Chung, Ka Young

AU - Dror, Ron O.

AU - Benovic, Jeffrey L.

PY - 2021/1/21

Y1 - 2021/1/21

N2 - Komolov et al. use structural, computational, and functional approaches to characterize the mechanism of calmodulin-mediated activation of the G protein-coupled receptor kinase GRK5. Additional findings reveal that GRK5 activation and localization cooperate under control of calmodulin for selective targeting of substrates at the plasma membrane and cytoplasm.

AB - Komolov et al. use structural, computational, and functional approaches to characterize the mechanism of calmodulin-mediated activation of the G protein-coupled receptor kinase GRK5. Additional findings reveal that GRK5 activation and localization cooperate under control of calmodulin for selective targeting of substrates at the plasma membrane and cytoplasm.

KW - G protein-coupled receptor

KW - X-ray crystallography

KW - calcium

KW - hydrogen/deuterium exchange mass spectrometry

KW - molecular dynamics

KW - phosphorylation

KW - protein kinase

UR - https://www.scopus.com/pages/publications/85099199951

U2 - 10.1016/j.molcel.2020.11.026

DO - 10.1016/j.molcel.2020.11.026

M3 - Article

C2 - 33321095

AN - SCOPUS:85099199951

SN - 1097-2765

VL - 81

SP - 323-339.e11

JO - Molecular Cell

JF - Molecular Cell

IS - 2

ER -

Structure of a GRK5-Calmodulin Complex Reveals Molecular Mechanism of GRK Activation and Substrate Targeting

Abstract

Keywords

UN SDGs

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