Structural modification of NADPH oxidase activator (Noxa 1) by oxidative stress: An experimental and computational study

Pankaj Attri; Jae Hyun Park; Joey De Backer; Myeongkyu Kim; Ji Hye Yun; Yunseok Heo; Sylvia Dewilde; Masaharu Shiratani; Eun Ha Choi; Weontae Lee; Annemie Bogaerts

doi:10.1016/j.ijbiomac.2020.09.120

Structural modification of NADPH oxidase activator (Noxa 1) by oxidative stress: An experimental and computational study

Pankaj Attri, Jae Hyun Park, Joey De Backer, Myeongkyu Kim, Ji Hye Yun, Yunseok Heo, Sylvia Dewilde, Masaharu Shiratani, Eun Ha Choi, Weontae Lee, Annemie Bogaerts

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

NADPH oxidases 1 (NOX1) derived reactive oxygen species (ROS) play an important role in the progression of cancer through signaling pathways. Therefore, in this paper, we demonstrate the effect of cold atmospheric plasma (CAP) on the structural changes of Noxa1 SH3 protein, one of the regulatory subunits of NOX1. For this purpose, firstly we purified the Noxa1 SH3 protein and analyzed the structure using X-ray crystallography, and subsequently, we treated the protein with two types of CAP reactors such as pulsed dielectric barrier discharge (DBD) and Soft Jet for different time intervals. The structural deformation of Noxa1 SH3 protein was analyzed by various experimental methods (circular dichroism, fluorescence, and NMR spectroscopy) and by MD simulations. Additionally, we demonstrate the effect of CAP (DBD and Soft Jet) on the viability and expression of NOX1 in A375 cancer cells. Our results are useful to understand the structural modification/oxidation occur in protein due to reactive oxygen and nitrogen (RONS) species generated by CAP.

Original language	English
Pages (from-to)	2405-2414
Number of pages	10
Journal	International Journal of Biological Macromolecules
Volume	163
DOIs	https://doi.org/10.1016/j.ijbiomac.2020.09.120
State	Published - 15 Nov 2020
Externally published	Yes

Keywords

MD simulations
Noxa1 SH3
Oxidative stress
X-ray crystallography

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ijbiomac.2020.09.120

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Attri, P., Park, J. H., De Backer, J., Kim, M., Yun, J. H., Heo, Y., Dewilde, S., Shiratani, M., Choi, E. H., Lee, W., & Bogaerts, A. (2020). Structural modification of NADPH oxidase activator (Noxa 1) by oxidative stress: An experimental and computational study. International Journal of Biological Macromolecules, 163, 2405-2414. https://doi.org/10.1016/j.ijbiomac.2020.09.120

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title = "Structural modification of NADPH oxidase activator (Noxa 1) by oxidative stress: An experimental and computational study",

abstract = "NADPH oxidases 1 (NOX1) derived reactive oxygen species (ROS) play an important role in the progression of cancer through signaling pathways. Therefore, in this paper, we demonstrate the effect of cold atmospheric plasma (CAP) on the structural changes of Noxa1 SH3 protein, one of the regulatory subunits of NOX1. For this purpose, firstly we purified the Noxa1 SH3 protein and analyzed the structure using X-ray crystallography, and subsequently, we treated the protein with two types of CAP reactors such as pulsed dielectric barrier discharge (DBD) and Soft Jet for different time intervals. The structural deformation of Noxa1 SH3 protein was analyzed by various experimental methods (circular dichroism, fluorescence, and NMR spectroscopy) and by MD simulations. Additionally, we demonstrate the effect of CAP (DBD and Soft Jet) on the viability and expression of NOX1 in A375 cancer cells. Our results are useful to understand the structural modification/oxidation occur in protein due to reactive oxygen and nitrogen (RONS) species generated by CAP.",

keywords = "MD simulations, Noxa1 SH3, Oxidative stress, X-ray crystallography",

author = "Pankaj Attri and Park, \{Jae Hyun\} and \{De Backer\}, Joey and Myeongkyu Kim and Yun, \{Ji Hye\} and Yunseok Heo and Sylvia Dewilde and Masaharu Shiratani and Choi, \{Eun Ha\} and Weontae Lee and Annemie Bogaerts",

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year = "2020",

month = nov,

day = "15",

doi = "10.1016/j.ijbiomac.2020.09.120",

language = "English",

volume = "163",

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T1 - Structural modification of NADPH oxidase activator (Noxa 1) by oxidative stress

T2 - An experimental and computational study

AU - Attri, Pankaj

AU - Park, Jae Hyun

AU - De Backer, Joey

AU - Kim, Myeongkyu

AU - Yun, Ji Hye

AU - Heo, Yunseok

AU - Dewilde, Sylvia

AU - Shiratani, Masaharu

AU - Choi, Eun Ha

AU - Lee, Weontae

AU - Bogaerts, Annemie

PY - 2020/11/15

Y1 - 2020/11/15

N2 - NADPH oxidases 1 (NOX1) derived reactive oxygen species (ROS) play an important role in the progression of cancer through signaling pathways. Therefore, in this paper, we demonstrate the effect of cold atmospheric plasma (CAP) on the structural changes of Noxa1 SH3 protein, one of the regulatory subunits of NOX1. For this purpose, firstly we purified the Noxa1 SH3 protein and analyzed the structure using X-ray crystallography, and subsequently, we treated the protein with two types of CAP reactors such as pulsed dielectric barrier discharge (DBD) and Soft Jet for different time intervals. The structural deformation of Noxa1 SH3 protein was analyzed by various experimental methods (circular dichroism, fluorescence, and NMR spectroscopy) and by MD simulations. Additionally, we demonstrate the effect of CAP (DBD and Soft Jet) on the viability and expression of NOX1 in A375 cancer cells. Our results are useful to understand the structural modification/oxidation occur in protein due to reactive oxygen and nitrogen (RONS) species generated by CAP.

AB - NADPH oxidases 1 (NOX1) derived reactive oxygen species (ROS) play an important role in the progression of cancer through signaling pathways. Therefore, in this paper, we demonstrate the effect of cold atmospheric plasma (CAP) on the structural changes of Noxa1 SH3 protein, one of the regulatory subunits of NOX1. For this purpose, firstly we purified the Noxa1 SH3 protein and analyzed the structure using X-ray crystallography, and subsequently, we treated the protein with two types of CAP reactors such as pulsed dielectric barrier discharge (DBD) and Soft Jet for different time intervals. The structural deformation of Noxa1 SH3 protein was analyzed by various experimental methods (circular dichroism, fluorescence, and NMR spectroscopy) and by MD simulations. Additionally, we demonstrate the effect of CAP (DBD and Soft Jet) on the viability and expression of NOX1 in A375 cancer cells. Our results are useful to understand the structural modification/oxidation occur in protein due to reactive oxygen and nitrogen (RONS) species generated by CAP.

KW - MD simulations

KW - Noxa1 SH3

KW - Oxidative stress

KW - X-ray crystallography

UR - https://www.scopus.com/pages/publications/85091677852

U2 - 10.1016/j.ijbiomac.2020.09.120

DO - 10.1016/j.ijbiomac.2020.09.120

M3 - Article

C2 - 32961197

AN - SCOPUS:85091677852

SN - 0141-8130

VL - 163

SP - 2405

EP - 2414

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

ER -

Structural modification of NADPH oxidase activator (Noxa 1) by oxidative stress: An experimental and computational study

Abstract

Keywords

UN SDGs

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