Structural and biochemical characterization of a carbohydrate acetylesterase from Sinorhizobium meliloti 1021

Kyungmin Kim; Bum Han Ryu; Sung Soo Kim; Deu Rae An; Tri Duc Ngo; Ramesh Pandian; Kyeong Kyu Kim; T. Doohun Kim

doi:10.1016/j.febslet.2014.11.033

Structural and biochemical characterization of a carbohydrate acetylesterase from Sinorhizobium meliloti 1021

Kyungmin Kim
, Bum Han Ryu
, Sung Soo Kim
, Deu Rae An
, Tri Duc Ngo
, Ramesh Pandian
, Kyeong Kyu Kim
, T. Doohun Kim

Sungkyunkwan University
Ajou University

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

In many microorganisms, carbohydrate acetylesterases remove the acetyl groups from various types of carbohydrates. Sm23 from Sinorhizobium meliloti is a putative member of carbohydrate esterase family 3 (CE3) in the CAZy classification system. Here, we determined the crystal structure of Sm23 at 1.75 Å resolution and investigated functional properties using biochemical methods. Furthermore, immobilized Sm23 exhibited improved stability compared with soluble Sm23, which can be used for the design of plant cell wall degrading-systems.

Original language	English
Pages (from-to)	117-122
Number of pages	6
Journal	FEBS Letters
Volume	589
Issue number	1
DOIs	https://doi.org/10.1016/j.febslet.2014.11.033
State	Published - 2 Feb 2015
Externally published	Yes

Keywords

Acetylation
Plant cell wall
Rhizobium species

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10.1016/j.febslet.2014.11.033

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title = "Structural and biochemical characterization of a carbohydrate acetylesterase from Sinorhizobium meliloti 1021",

abstract = "In many microorganisms, carbohydrate acetylesterases remove the acetyl groups from various types of carbohydrates. Sm23 from Sinorhizobium meliloti is a putative member of carbohydrate esterase family 3 (CE3) in the CAZy classification system. Here, we determined the crystal structure of Sm23 at 1.75 {\AA} resolution and investigated functional properties using biochemical methods. Furthermore, immobilized Sm23 exhibited improved stability compared with soluble Sm23, which can be used for the design of plant cell wall degrading-systems.",

keywords = "Acetylation, Plant cell wall, Rhizobium species",

author = "Kyungmin Kim and Ryu, \{Bum Han\} and Kim, \{Sung Soo\} and An, \{Deu Rae\} and Ngo, \{Tri Duc\} and Ramesh Pandian and Kim, \{Kyeong Kyu\} and Kim, \{T. Doohun\}",

note = "Publisher Copyright: {\textcopyright} 2014 Federation of European Biochemical Societies.",

year = "2015",

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doi = "10.1016/j.febslet.2014.11.033",

language = "English",

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journal = "FEBS Letters",

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T1 - Structural and biochemical characterization of a carbohydrate acetylesterase from Sinorhizobium meliloti 1021

AU - Kim, Kyungmin

AU - Ryu, Bum Han

AU - Kim, Sung Soo

AU - An, Deu Rae

AU - Ngo, Tri Duc

AU - Pandian, Ramesh

AU - Kim, Kyeong Kyu

AU - Kim, T. Doohun

PY - 2015/2/2

Y1 - 2015/2/2

N2 - In many microorganisms, carbohydrate acetylesterases remove the acetyl groups from various types of carbohydrates. Sm23 from Sinorhizobium meliloti is a putative member of carbohydrate esterase family 3 (CE3) in the CAZy classification system. Here, we determined the crystal structure of Sm23 at 1.75 Å resolution and investigated functional properties using biochemical methods. Furthermore, immobilized Sm23 exhibited improved stability compared with soluble Sm23, which can be used for the design of plant cell wall degrading-systems.

AB - In many microorganisms, carbohydrate acetylesterases remove the acetyl groups from various types of carbohydrates. Sm23 from Sinorhizobium meliloti is a putative member of carbohydrate esterase family 3 (CE3) in the CAZy classification system. Here, we determined the crystal structure of Sm23 at 1.75 Å resolution and investigated functional properties using biochemical methods. Furthermore, immobilized Sm23 exhibited improved stability compared with soluble Sm23, which can be used for the design of plant cell wall degrading-systems.

KW - Acetylation

KW - Plant cell wall

KW - Rhizobium species

UR - https://www.scopus.com/pages/publications/84918789818

U2 - 10.1016/j.febslet.2014.11.033

DO - 10.1016/j.febslet.2014.11.033

M3 - Article

C2 - 25436419

AN - SCOPUS:84918789818

SN - 0014-5793

VL - 589

SP - 117

EP - 122

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Structural and biochemical characterization of a carbohydrate acetylesterase from Sinorhizobium meliloti 1021

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Keywords

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