@inbook{a642e859b1c14915bc2af92f74f5692b,
title = "Stable Isotope Tracing and Metabolomics to Study In Vivo Brown Adipose Tissue Metabolic Fluxes",
abstract = "Brown adipose tissue (BAT) demonstrates extraordinary metabolic capacity. Previous research using conventional radio tracers reveals that BAT can act as a sink for a diverse menu of nutrients; still, the question of how BAT utilizes these nutrients remains unclear. Recent advances in mass spectrometry (MS) coupled to stable isotope tracing methods have greatly improved our understanding of metabolism in biology. Here, we have developed a BAT-tailored metabolomics and stable isotope tracing protocol using, as an example, the universally labeled13C-glucose, a key nutrient heavily utilized by BAT. This method enables metabolic roadmaps to be drawn and pathway fluxes to be inferred for each nutrient tracer within BAT and its application could uncover new metabolic pathways not previously appreciated for BAT physiology.",
keywords = "Brown adipose tissue, Brown fat, Gavage, Glucose, Liquid chromatography-mass spectrometry (LC-MS), Metabolism, Metabolomics, Stable isotope tracing, Temperature acclimation",
author = "Jung, \{Su Myung\} and Johnny Le and Doxsey, \{Will G.\} and Haley, \{John A.\} and Grace Park and Guertin, \{David A.\} and Cholsoon Jang",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2087-8\_8",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "119--130",
booktitle = "Methods in Molecular Biology",
}