Spirosoma metallilatum sp. nov., isolated from an automotive air conditioning system

A Gram-stain-negative, aerobic, rod-shaped and yellow-pigmented bacterial strain, designated TX0405^T, was isolated from an automotive air conditioning system. Colonies were circular, convex, semi-translucent, smooth and yellow. The strain grew at 20–28ºC (optimum, 28ºC), at pH 6.0–7.5 (optimum, pH 6.5) and in the presence of 0–1% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence indicated that the strain was grouped with the members of the genus Spirosoma, with the sequence similarities of 93.0 and 92.3% with Spirosoma panaciterrae DSM 21099^T and Spirosoma swuense JBM2-3^T, respectively. The major fatty acids of the strain were summed feature 3 (C_{16: 1}ω7c and/or C_{16: 1} ω6c) (33.2 %), C_{16: 1}ω5c (25.4 %), iso-C_{15: 0} (15.0 %), C_{16: 0} (6.5 %) and iso-C_{17: 0} 3-OH (6.2 %). The predominant menaquinone was MK-7. The polar lipids were phosphatidylethanolamine, an unidentified aminolipid, two unidentified aminophospholipids and three unidentified lipids. The DNA G+C content of the type strain was 51.9 mol%. On the basis of the data presented, strain TX0405T represents a novel species of the genus Spirosoma, for which the name Spirosoma metallilatum sp. nov. (=KACC 19012^T=NBRC 112493^T) is proposed.