Spirosoma carri sp. nov., isolated from an automobile air conditioning system

A Gram-stain-negative and yellow-pigmented bacterial strain, designated TX0406^T, was isolated from an automobile evaporator core collected in Korea. The cells were non-motile, aerobic and rod-shaped. The strain grew at 15-37 °C (optimum, 25 °C), at pH 6.0-7.0 (optimum, 6.5) and in the presence of 0-1.5 % (w/v) NaCl. Phylogenetically, the strain was related to members of the genus Spirosoma (93.7-90.7 % 16S rRNA sequence similarities) and showed the highest sequence similarity of 93.7 % to Spirosomapulveris JSH5-14^T. The major fatty acids of the strain were summed feature 3 (C_16:1ω7c and/or C_16:1ω6c), C_16:1ω5c and C_16:0. The predominant menaquinone was MK-7. The polar lipid profile revealed the presence of phosphatidylethanolamine, an unidentified aminolipid, unidentified aminophospholipids and unidentified lipids. The DNA G+C content of the strain was 58.7 mol%. Based on phenotypic, genotypic and chemotaxonomic data, strain TX0406^Trepresents a novel species in the genus Spirosoma, for which the name Spirosoma carri sp. nov. (=KACC 19013^T=NBRC 112494^T) is proposed.