TY - JOUR
T1 - Somatic copy number alterations associated with Japanese or endometriosis in ovarian clear cell adenocarcinoma
AU - Okamoto, Aikou
AU - Sehouli, Jalid
AU - Yanaihara, Nozomu
AU - Hirata, Yukihiro
AU - Braicu, Ioana
AU - Kim, Byoung Gie
AU - Takakura, Satoshi
AU - Saito, Misato
AU - Yanagida, Satoshi
AU - Takenaka, Masataka
AU - Yamaguchi, Noriko
AU - Morikawa, Asuka
AU - Tanabe, Hiroshi
AU - Yamada, Kyosuke
AU - Yoshihara, Kosuke
AU - Enomoto, Takayuki
AU - Itamochi, Hiroaki
AU - Kigawa, Junzo
AU - Matsumura, Noriomi
AU - Konishi, Ikuo
AU - Aida, Satoshi
AU - Aoki, Yuko
AU - Ishii, Nobuya
AU - Ochiai, Kazunori
AU - Akiyama, Tetsu
AU - Urashima, Mitsuyoshi
N1 - Publisher Copyright:
© 2015 Okamoto et al.
PY - 2015/2/6
Y1 - 2015/2/6
N2 - When compared with other epithelial ovarian cancers, the clinical characteristics of ovarian clear cell adenocarcinoma (CCC) include 1) a higher incidence among Japanese, 2) an association with endometriosis, 3) poor prognosis in advanced stages, and 4) a higher incidence of thrombosis as a complication. We used high resolution comparative genomic hybridization (CGH) to identify somatic copy number alterations (SCNAs) associated with each of these clinical characteristics of CCC. The Human Genome CGH 244A Oligo Microarray was used to examine 144 samples obtained from 120 Japanese, 15 Korean, and nine German patients with CCC. The entire 8q chromosome (minimum corrected p-value: q = 0.0001) and chromosome 20q13.2 including the ZNF217 locus (q = 0.0078) were amplified significantly more in Japanese than in Korean or German samples. This copy number amplification of the ZNF217 gene was confirmed by quantitative real-time polymerase chain reaction (Q-PCR). ZNF217 RNA levels were also higher in Japanese tumor samples than in non-Japanese samples (P = 0.027). Moreover, endometriosis was associated with amplification of EGFR gene (q = 0.047), which was again confirmed by Q-PCR and correlated with EGFR RNA expression. However, no SCNAs were significantly associated with prognosis or thrombosis. These results indicated that there may be an association between CCC and ZNF217 amplification among Japanese patients as well as between endometriosis and EGFR gene amplifications.
AB - When compared with other epithelial ovarian cancers, the clinical characteristics of ovarian clear cell adenocarcinoma (CCC) include 1) a higher incidence among Japanese, 2) an association with endometriosis, 3) poor prognosis in advanced stages, and 4) a higher incidence of thrombosis as a complication. We used high resolution comparative genomic hybridization (CGH) to identify somatic copy number alterations (SCNAs) associated with each of these clinical characteristics of CCC. The Human Genome CGH 244A Oligo Microarray was used to examine 144 samples obtained from 120 Japanese, 15 Korean, and nine German patients with CCC. The entire 8q chromosome (minimum corrected p-value: q = 0.0001) and chromosome 20q13.2 including the ZNF217 locus (q = 0.0078) were amplified significantly more in Japanese than in Korean or German samples. This copy number amplification of the ZNF217 gene was confirmed by quantitative real-time polymerase chain reaction (Q-PCR). ZNF217 RNA levels were also higher in Japanese tumor samples than in non-Japanese samples (P = 0.027). Moreover, endometriosis was associated with amplification of EGFR gene (q = 0.047), which was again confirmed by Q-PCR and correlated with EGFR RNA expression. However, no SCNAs were significantly associated with prognosis or thrombosis. These results indicated that there may be an association between CCC and ZNF217 amplification among Japanese patients as well as between endometriosis and EGFR gene amplifications.
UR - https://www.scopus.com/pages/publications/84922738083
U2 - 10.1371/journal.pone.0116977
DO - 10.1371/journal.pone.0116977
M3 - Article
C2 - 25658832
AN - SCOPUS:84922738083
SN - 1932-6203
VL - 10
JO - PLoS ONE
JF - PLoS ONE
IS - 2
M1 - e0116977
ER -
