Solid-phase colorimetric apta-biosensor for thrombin detection

Wonjung Lee; Samy M. Shaban; Do Gi Pyun; Dong Hwan Kim

doi:10.1016/j.tsf.2019.137428

Solid-phase colorimetric apta-biosensor for thrombin detection

Wonjung Lee
, Samy M. Shaban
, Do Gi Pyun
, Dong Hwan Kim

Department of Chemical Engineering

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Herein, we developed a solid-phase colorimetric apta-biosensor for thrombin detection. Aggregation of gold nanoparticles (AuNPs) deposited on a 3-aminopropyltriethoxysilane (APTES) substrate was utilized to form the colorimetric sensor. The APTES-coated substrate with primary aptamer (TBA1)-conjugated AuNPs (AuNP-TBA1) was used to construct the solid-phase sensing platform, where an enhancement of the localized surface plasmon resonance due to the AuNPs was observed upon incubation with a target molecule (thrombin). The introduction of secondary aptamer-conjugated AuNPs (AuNP-TBA2) induced plasmonic coupling of the AuNPs, resulting in significantly enhanced spectral signals and peak shifts and visually detectable color changes. This sensing platform not only includes several advantages, such as simplicity and rapid detection, but also allows naked-eye detection sensitivity at 3 μg/mL with no analytical equipment and a limit of detection of 1.33 μg/mL using UV–vis.

Original language	English
Article number	137428
Journal	Thin Solid Films
Volume	686
DOIs	https://doi.org/10.1016/j.tsf.2019.137428
State	Published - 30 Sep 2019

Keywords

Aptamer
Colorimetric biosensor
Gold
Nanoparticles
Solid-phase detection
Thrombin

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title = "Solid-phase colorimetric apta-biosensor for thrombin detection",

abstract = "Herein, we developed a solid-phase colorimetric apta-biosensor for thrombin detection. Aggregation of gold nanoparticles (AuNPs) deposited on a 3-aminopropyltriethoxysilane (APTES) substrate was utilized to form the colorimetric sensor. The APTES-coated substrate with primary aptamer (TBA1)-conjugated AuNPs (AuNP-TBA1) was used to construct the solid-phase sensing platform, where an enhancement of the localized surface plasmon resonance due to the AuNPs was observed upon incubation with a target molecule (thrombin). The introduction of secondary aptamer-conjugated AuNPs (AuNP-TBA2) induced plasmonic coupling of the AuNPs, resulting in significantly enhanced spectral signals and peak shifts and visually detectable color changes. This sensing platform not only includes several advantages, such as simplicity and rapid detection, but also allows naked-eye detection sensitivity at 3 μg/mL with no analytical equipment and a limit of detection of 1.33 μg/mL using UV–vis.",

keywords = "Aptamer, Colorimetric biosensor, Gold, Nanoparticles, Solid-phase detection, Thrombin",

author = "Wonjung Lee and Shaban, \{Samy M.\} and Pyun, \{Do Gi\} and Kim, \{Dong Hwan\}",

note = "Publisher Copyright: {\textcopyright} 2019 Elsevier B.V.",

year = "2019",

month = sep,

day = "30",

doi = "10.1016/j.tsf.2019.137428",

language = "English",

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journal = "Thin Solid Films",

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TY - JOUR

T1 - Solid-phase colorimetric apta-biosensor for thrombin detection

AU - Lee, Wonjung

AU - Shaban, Samy M.

AU - Pyun, Do Gi

AU - Kim, Dong Hwan

PY - 2019/9/30

Y1 - 2019/9/30

N2 - Herein, we developed a solid-phase colorimetric apta-biosensor for thrombin detection. Aggregation of gold nanoparticles (AuNPs) deposited on a 3-aminopropyltriethoxysilane (APTES) substrate was utilized to form the colorimetric sensor. The APTES-coated substrate with primary aptamer (TBA1)-conjugated AuNPs (AuNP-TBA1) was used to construct the solid-phase sensing platform, where an enhancement of the localized surface plasmon resonance due to the AuNPs was observed upon incubation with a target molecule (thrombin). The introduction of secondary aptamer-conjugated AuNPs (AuNP-TBA2) induced plasmonic coupling of the AuNPs, resulting in significantly enhanced spectral signals and peak shifts and visually detectable color changes. This sensing platform not only includes several advantages, such as simplicity and rapid detection, but also allows naked-eye detection sensitivity at 3 μg/mL with no analytical equipment and a limit of detection of 1.33 μg/mL using UV–vis.

AB - Herein, we developed a solid-phase colorimetric apta-biosensor for thrombin detection. Aggregation of gold nanoparticles (AuNPs) deposited on a 3-aminopropyltriethoxysilane (APTES) substrate was utilized to form the colorimetric sensor. The APTES-coated substrate with primary aptamer (TBA1)-conjugated AuNPs (AuNP-TBA1) was used to construct the solid-phase sensing platform, where an enhancement of the localized surface plasmon resonance due to the AuNPs was observed upon incubation with a target molecule (thrombin). The introduction of secondary aptamer-conjugated AuNPs (AuNP-TBA2) induced plasmonic coupling of the AuNPs, resulting in significantly enhanced spectral signals and peak shifts and visually detectable color changes. This sensing platform not only includes several advantages, such as simplicity and rapid detection, but also allows naked-eye detection sensitivity at 3 μg/mL with no analytical equipment and a limit of detection of 1.33 μg/mL using UV–vis.

KW - Aptamer

KW - Colorimetric biosensor

KW - Gold

KW - Nanoparticles

KW - Solid-phase detection

KW - Thrombin

UR - https://www.scopus.com/pages/publications/85069633418

U2 - 10.1016/j.tsf.2019.137428

DO - 10.1016/j.tsf.2019.137428

M3 - Article

AN - SCOPUS:85069633418

SN - 0040-6090

VL - 686

JO - Thin Solid Films

JF - Thin Solid Films

M1 - 137428

ER -

Solid-phase colorimetric apta-biosensor for thrombin detection

Abstract

Keywords

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