Single-walled carbon nanotube spectroscopy in live cells: Towards long-term labels and optical sensors

Daniel A. Heller; Seunghyun Baik; Thomas E. Eurell; Michael S. Strano

doi:10.1002/adma.200500477

Single-walled carbon nanotube spectroscopy in live cells: Towards long-term labels and optical sensors

Daniel A. Heller
, Seunghyun Baik
, Thomas E. Eurell
, Michael S. Strano

Department of Mechanical Engineering

University of Illinois at Urbana-Champaign

Research output: Contribution to journal › Article › peer-review

476 Scopus citations

Abstract

The application of single-walled carbon nanotubes encapsulated by a DNA oligonucleotides (DNA-SWNT) as photo-bleaching resistant, non-quenching markers which remained functional in live cells were investigated. Raman scattering and fluorescence spectra of DNA-SWNT were measured from within live as well as hematoxylin and eosin stained cells, showing continued emission after staining and spectral changes upon uptake. Nanotubes were suspended with a 30-base alternating GT sequence of single-stranded DNA. Nanotubes were found to incorporate into cytoplasmic vesicles and labeled the prenuclear region of the cells, but did not enter the nuclear envelope.

Original language	English
Pages (from-to)	2793-2799
Number of pages	7
Journal	Advanced Materials
Volume	17
Issue number	23
DOIs	https://doi.org/10.1002/adma.200500477
State	Published - 5 Dec 2005

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abstract = "The application of single-walled carbon nanotubes encapsulated by a DNA oligonucleotides (DNA-SWNT) as photo-bleaching resistant, non-quenching markers which remained functional in live cells were investigated. Raman scattering and fluorescence spectra of DNA-SWNT were measured from within live as well as hematoxylin and eosin stained cells, showing continued emission after staining and spectral changes upon uptake. Nanotubes were suspended with a 30-base alternating GT sequence of single-stranded DNA. Nanotubes were found to incorporate into cytoplasmic vesicles and labeled the prenuclear region of the cells, but did not enter the nuclear envelope.",

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year = "2005",

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T2 - Towards long-term labels and optical sensors

AU - Heller, Daniel A.

AU - Baik, Seunghyun

AU - Eurell, Thomas E.

AU - Strano, Michael S.

PY - 2005/12/5

Y1 - 2005/12/5

N2 - The application of single-walled carbon nanotubes encapsulated by a DNA oligonucleotides (DNA-SWNT) as photo-bleaching resistant, non-quenching markers which remained functional in live cells were investigated. Raman scattering and fluorescence spectra of DNA-SWNT were measured from within live as well as hematoxylin and eosin stained cells, showing continued emission after staining and spectral changes upon uptake. Nanotubes were suspended with a 30-base alternating GT sequence of single-stranded DNA. Nanotubes were found to incorporate into cytoplasmic vesicles and labeled the prenuclear region of the cells, but did not enter the nuclear envelope.

AB - The application of single-walled carbon nanotubes encapsulated by a DNA oligonucleotides (DNA-SWNT) as photo-bleaching resistant, non-quenching markers which remained functional in live cells were investigated. Raman scattering and fluorescence spectra of DNA-SWNT were measured from within live as well as hematoxylin and eosin stained cells, showing continued emission after staining and spectral changes upon uptake. Nanotubes were suspended with a 30-base alternating GT sequence of single-stranded DNA. Nanotubes were found to incorporate into cytoplasmic vesicles and labeled the prenuclear region of the cells, but did not enter the nuclear envelope.

UR - https://www.scopus.com/pages/publications/29144458221

U2 - 10.1002/adma.200500477

DO - 10.1002/adma.200500477

M3 - Article

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JO - Advanced Materials

JF - Advanced Materials

IS - 23

ER -

Single-walled carbon nanotube spectroscopy in live cells: Towards long-term labels and optical sensors

Abstract

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