Shewanella litorisediminis sp. nov., a gammaproteobacterium isolated from a tidal flat sediment

A Gram-negative, facultatively anaerobic, non-motile and rod-shaped bacterial strain, designated SMK1-12T,was isolated from a tidal flat sediment on the western coast of Korea. Phylogenetic analyses based on 16S rRNA and gyrB gene sequences showed that strain SMK1-12T belonged to the genus Shewanella, clustering with the type strain of Shewanella amazonensis. Strain SMK1-12T exhibited the highest 16S rRNA gene sequence similarity value (97.0 %) and the highest gyrB sequence similarity value (87.8 %) to S. amazonensis SB2BT, respectively. StrainSMK1-12T contained simultaneously both menaquinones and ubiquinones; the predominant menaquinone was MK-7 and the predominant ubiquinones were Q-7 and Q-8. The major fatty acids ([10 % of the total fatty acids) detected in strain SMK1-12T were the MIDI system summed feature 3 (iso-C15:0 2-OH and/or C16:1 x7c), iso-C15:0, C17:1 x8c and C16:0. The DNA G-C content of strain SMK1-12T was 58.0 mol% and its mean DNA-DNA relatedness value with S. amazonensis ATCC 700329T was 15 ± 4.6 %.Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain SMK1-12T is distinguishable from recognized Shewanella species. On the basis of the data presented, strain SMK1-12T is considered to represent a novel Shewanella species, for which the name Shewanella litorisediminis sp. nov. is proposed. The type strain is SMK1-12T (=KCTC 23961T = CCUG 62411T).