Saucerneol D, a naturally occurring sesquilignan, inhibits LPS-induced iNOS expression in RAW264.7 cells by blocking NF-κB and MAPK activation

Tae Chul Moon; Jin Cheul Kim; Seung Eun Song; Seok Jong Suh; Chang Seob Seo; Youn Kyung Kim; Meihua Jin; Ju Hye Yang; Jong Keun Son; Yurngdong Jahng; Cheorl Ho Kim; Hyeun Wook Chang

doi:10.1016/j.intimp.2008.05.009

Saucerneol D, a naturally occurring sesquilignan, inhibits LPS-induced iNOS expression in RAW264.7 cells by blocking NF-κB and MAPK activation

Tae Chul Moon, Jin Cheul Kim, Seung Eun Song, Seok Jong Suh, Chang Seob Seo, Youn Kyung Kim, Meihua Jin, Ju Hye Yang, Jong Keun Son, Yurngdong Jahng, Cheorl Ho Kim, Hyeun Wook Chang

Department of Biological Sciences

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

We evaluated the ability of saucerneol D (SD), a tetrahydrofuran-type sesquilignan isolated from Saururus chinensis, to regulate the expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-stimulated murine macrophage-like RAW264.7 cells. SD consistently inhibited nitric oxide (NO) production in a dose-dependent manner, with an IC₅₀ of 2.62 μM, and also blocked LPS-induced iNOS expression. SD potently suppressed both the reporter gene expression and DNA-binding activity of nuclear factor-κB (NF-κB). In addition, SD inhibited IκB-α degradation in a concentration- and time-dependent manner. SD also inhibited LPS-induced activation of various mitogen-activated protein kinases, including extracellular signal-regulated kinase 1/2 (ERK1/2) and c-Jun NH₂-terminal kinase (JNK). These findings suggest that SD may inhibit LPS-induced iNOS expression by blocking NF-κB and MAPK activation.

Original language	English
Pages (from-to)	1395-1400
Number of pages	6
Journal	International Immunopharmacology
Volume	8
Issue number	10
DOIs	https://doi.org/10.1016/j.intimp.2008.05.009
State	Published - Oct 2008

Keywords

Electrophoretic mobility shift analysis;
Inducible nitric oxide synthase;
Mitogen-activated protein kinases
NF-κB;
Saucerneol D;
Saururus chinensis;

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10.1016/j.intimp.2008.05.009

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Moon, T. C., Kim, J. C., Song, S. E., Suh, S. J., Seo, C. S., Kim, Y. K., Jin, M., Yang, J. H., Son, J. K., Jahng, Y., Kim, C. H., & Chang, H. W. (2008). Saucerneol D, a naturally occurring sesquilignan, inhibits LPS-induced iNOS expression in RAW264.7 cells by blocking NF-κB and MAPK activation. International Immunopharmacology, 8(10), 1395-1400. https://doi.org/10.1016/j.intimp.2008.05.009

@article{0aa975dc36ba4021b2cb0e32288c2d26,

title = "Saucerneol D, a naturally occurring sesquilignan, inhibits LPS-induced iNOS expression in RAW264.7 cells by blocking NF-κB and MAPK activation",

abstract = "We evaluated the ability of saucerneol D (SD), a tetrahydrofuran-type sesquilignan isolated from Saururus chinensis, to regulate the expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-stimulated murine macrophage-like RAW264.7 cells. SD consistently inhibited nitric oxide (NO) production in a dose-dependent manner, with an IC50 of 2.62 μM, and also blocked LPS-induced iNOS expression. SD potently suppressed both the reporter gene expression and DNA-binding activity of nuclear factor-κB (NF-κB). In addition, SD inhibited IκB-α degradation in a concentration- and time-dependent manner. SD also inhibited LPS-induced activation of various mitogen-activated protein kinases, including extracellular signal-regulated kinase 1/2 (ERK1/2) and c-Jun NH2-terminal kinase (JNK). These findings suggest that SD may inhibit LPS-induced iNOS expression by blocking NF-κB and MAPK activation.",

keywords = "Electrophoretic mobility shift analysis;, Inducible nitric oxide synthase;, Mitogen-activated protein kinases, NF-κB;, Saucerneol D;, Saururus chinensis;",

author = "Moon, \{Tae Chul\} and Kim, \{Jin Cheul\} and Song, \{Seung Eun\} and Suh, \{Seok Jong\} and Seo, \{Chang Seob\} and Kim, \{Youn Kyung\} and Meihua Jin and Yang, \{Ju Hye\} and Son, \{Jong Keun\} and Yurngdong Jahng and Kim, \{Cheorl Ho\} and Chang, \{Hyeun Wook\}",

year = "2008",

month = oct,

doi = "10.1016/j.intimp.2008.05.009",

language = "English",

volume = "8",

pages = "1395--1400",

journal = "International Immunopharmacology",

issn = "1567-5769",

publisher = "Elsevier B.V.",

number = "10",

}

Moon, TC, Kim, JC, Song, SE, Suh, SJ, Seo, CS, Kim, YK, Jin, M, Yang, JH, Son, JK, Jahng, Y, Kim, CH & Chang, HW 2008, 'Saucerneol D, a naturally occurring sesquilignan, inhibits LPS-induced iNOS expression in RAW264.7 cells by blocking NF-κB and MAPK activation', International Immunopharmacology, vol. 8, no. 10, pp. 1395-1400. https://doi.org/10.1016/j.intimp.2008.05.009

TY - JOUR

T1 - Saucerneol D, a naturally occurring sesquilignan, inhibits LPS-induced iNOS expression in RAW264.7 cells by blocking NF-κB and MAPK activation

AU - Moon, Tae Chul

AU - Kim, Jin Cheul

AU - Song, Seung Eun

AU - Suh, Seok Jong

AU - Seo, Chang Seob

AU - Kim, Youn Kyung

AU - Jin, Meihua

AU - Yang, Ju Hye

AU - Son, Jong Keun

AU - Jahng, Yurngdong

AU - Kim, Cheorl Ho

AU - Chang, Hyeun Wook

PY - 2008/10

Y1 - 2008/10

N2 - We evaluated the ability of saucerneol D (SD), a tetrahydrofuran-type sesquilignan isolated from Saururus chinensis, to regulate the expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-stimulated murine macrophage-like RAW264.7 cells. SD consistently inhibited nitric oxide (NO) production in a dose-dependent manner, with an IC50 of 2.62 μM, and also blocked LPS-induced iNOS expression. SD potently suppressed both the reporter gene expression and DNA-binding activity of nuclear factor-κB (NF-κB). In addition, SD inhibited IκB-α degradation in a concentration- and time-dependent manner. SD also inhibited LPS-induced activation of various mitogen-activated protein kinases, including extracellular signal-regulated kinase 1/2 (ERK1/2) and c-Jun NH2-terminal kinase (JNK). These findings suggest that SD may inhibit LPS-induced iNOS expression by blocking NF-κB and MAPK activation.

AB - We evaluated the ability of saucerneol D (SD), a tetrahydrofuran-type sesquilignan isolated from Saururus chinensis, to regulate the expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-stimulated murine macrophage-like RAW264.7 cells. SD consistently inhibited nitric oxide (NO) production in a dose-dependent manner, with an IC50 of 2.62 μM, and also blocked LPS-induced iNOS expression. SD potently suppressed both the reporter gene expression and DNA-binding activity of nuclear factor-κB (NF-κB). In addition, SD inhibited IκB-α degradation in a concentration- and time-dependent manner. SD also inhibited LPS-induced activation of various mitogen-activated protein kinases, including extracellular signal-regulated kinase 1/2 (ERK1/2) and c-Jun NH2-terminal kinase (JNK). These findings suggest that SD may inhibit LPS-induced iNOS expression by blocking NF-κB and MAPK activation.

KW - Electrophoretic mobility shift analysis;

KW - Inducible nitric oxide synthase;

KW - Mitogen-activated protein kinases

KW - NF-κB;

KW - Saucerneol D;

KW - Saururus chinensis;

UR - https://www.scopus.com/pages/publications/50949086036

U2 - 10.1016/j.intimp.2008.05.009

DO - 10.1016/j.intimp.2008.05.009

M3 - Article

C2 - 18687301

AN - SCOPUS:50949086036

SN - 1567-5769

VL - 8

SP - 1395

EP - 1400

JO - International Immunopharmacology

JF - International Immunopharmacology

IS - 10

ER -

Saucerneol D, a naturally occurring sesquilignan, inhibits LPS-induced iNOS expression in RAW264.7 cells by blocking NF-κB and MAPK activation

Abstract

Keywords

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