Roseovarius aestuariivivens sp. Nov., isolated from a tidal flat

A Gram-stain-negative, non-motile, aerobic and coccoid, ovoid or rod-shaped bacterium, designated GHTF-24^T, was isolated from a tidal flat on the South Sea, South Korea, and subjected to polyphasic taxonomic study. The novel strain grew optimally at 30^ºC, at pH 7.0-8.0 and in the presence of 2.0-3.0 % (w/v) NaCl. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain GHTF-24^T fell within the clade comprising the type strains of species of the genus Roseovarius. Strain GHTF-24^T exhibited 16S rRNA gene sequence similarity values of 97.0 % to each of Roseovarius halotolerans HJ50^T and Roseovarius pacificus 81-2^T and of 91.6-96.5 % to the type strains of the other species of the genus Roseovarius. Strain GHTF-24^T contained Q-10 as the predominant ubiquinone and C_18:1ω7c as the major fatty acid. The major polar lipids of strain GHTF-24^T were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain GHTF-24^T was 62.3 mol%, and its mean DNA-DNA relatedness values with the type strains of R. halotolerans and R. pacificus were 13-16 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain GHTF-24^T is separated from other recognized species of the genus Roseovarius. On the basis of the data presented, strain GHTF-24^T is considered to represent a novel species of the genus Roseovarius, for which the name Roseovarius aestuariivivens sp. nov. is proposed. The type strain is GHTF-24^T (=KCTC 52454^T=NBRC 112420^T).