Reyranella terrae sp. Nov., isolated from an agricultural soil, and emended description of the genus Reyranella

A novel strain designated 11G32^T was isolated from an agricultural soil cultivated with Chinese cabbage in Korea. The cells were Gram-stain-negative, aerobic, non-motile and rod-shaped. The strain grew at 15-28 °C (optimum, 20 °C), pH 5.0-7.0 (optimum, pH 5.0-6.0) and without NaCl. Phylogenetically, the strain was found to be closely related to members of the genus Reyranella and showed 16S rRNA gene sequence similarities of 97.18, 96.76 and 95.99% with Reyranella graminifolii Wo-34^T, Reyranella massiliensis 521^T and Reyranella soli KIS14-15^T, respectively. The major fatty acids were C19:0 cyclo ω8c, C16:0, summed feature 8 (C_18:1ω7c and/or C_18:1ω6c) and 11-methyl C_18:1ω7c. The predominant ubiquinone was Q-10. The polar lipids profile revealed the presence of phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, unidentified aminolipids, unidentified phospholipids and unidentified lipids. On the basis of data presented, strain 11G32^T is considered to represent a novel species of the genus Reyranella, for which the name Reyranella terrae sp. nov. is proposed. The type strain is 11G32^T (=KACC 18486^T=NBRC 111476^T).