Restoration of growth phenotypes of Escherichia coli DH5α in minimal media through reversal of a point mutation in purB

Suk Chae Jung; Chris L. Smith; Ki Sung Lee; Min Eui Hong; Dae Hyuk Kweon; Gregory Stephanopoulos; Yong Su Jin

doi:10.1128/AEM.01210-10

Restoration of growth phenotypes of Escherichia coli DH5α in minimal media through reversal of a point mutation in purB

Suk Chae Jung
, Chris L. Smith
, Ki Sung Lee
, Min Eui Hong
, Dae Hyuk Kweon
, Gregory Stephanopoulos
, Yong Su Jin

Department of Integrative Biotechnology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

A point mutation (E115K) resulting in slower growth of Escherichia coli DH5α and XL1-Blue in minimal media was identified in the purB gene, coding for adenylosuccinate lyase (ASL), through complementation with an E. coli K-12 genomic library and serial subcultures. Chromosomal modification reversing the mutation to the wild type restored growth phenotypes in minimal media.

Original language	English
Pages (from-to)	6307-6309
Number of pages	3
Journal	Applied and Environmental Microbiology
Volume	76
Issue number	18
DOIs	https://doi.org/10.1128/AEM.01210-10
State	Published - Sep 2010

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title = "Restoration of growth phenotypes of Escherichia coli DH5α in minimal media through reversal of a point mutation in purB",

abstract = "A point mutation (E115K) resulting in slower growth of Escherichia coli DH5α and XL1-Blue in minimal media was identified in the purB gene, coding for adenylosuccinate lyase (ASL), through complementation with an E. coli K-12 genomic library and serial subcultures. Chromosomal modification reversing the mutation to the wild type restored growth phenotypes in minimal media.",

author = "Jung, \{Suk Chae\} and Smith, \{Chris L.\} and Lee, \{Ki Sung\} and Hong, \{Min Eui\} and Kweon, \{Dae Hyuk\} and Gregory Stephanopoulos and Jin, \{Yong Su\}",

year = "2010",

month = sep,

doi = "10.1128/AEM.01210-10",

language = "English",

volume = "76",

pages = "6307--6309",

journal = "Applied and Environmental Microbiology",

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T1 - Restoration of growth phenotypes of Escherichia coli DH5α in minimal media through reversal of a point mutation in purB

AU - Jung, Suk Chae

AU - Smith, Chris L.

AU - Lee, Ki Sung

AU - Hong, Min Eui

AU - Kweon, Dae Hyuk

AU - Stephanopoulos, Gregory

AU - Jin, Yong Su

PY - 2010/9

Y1 - 2010/9

N2 - A point mutation (E115K) resulting in slower growth of Escherichia coli DH5α and XL1-Blue in minimal media was identified in the purB gene, coding for adenylosuccinate lyase (ASL), through complementation with an E. coli K-12 genomic library and serial subcultures. Chromosomal modification reversing the mutation to the wild type restored growth phenotypes in minimal media.

AB - A point mutation (E115K) resulting in slower growth of Escherichia coli DH5α and XL1-Blue in minimal media was identified in the purB gene, coding for adenylosuccinate lyase (ASL), through complementation with an E. coli K-12 genomic library and serial subcultures. Chromosomal modification reversing the mutation to the wild type restored growth phenotypes in minimal media.

UR - https://www.scopus.com/pages/publications/77956793141

U2 - 10.1128/AEM.01210-10

DO - 10.1128/AEM.01210-10

M3 - Article

C2 - 20675450

AN - SCOPUS:77956793141

SN - 0099-2240

VL - 76

SP - 6307

EP - 6309

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 18

ER -

Restoration of growth phenotypes of Escherichia coli DH5α in minimal media through reversal of a point mutation in purB

Abstract

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