Redox regulation of the tumor suppressor PTEN by glutathione

Yujeong Kim; Yong Bhum Song; Tae Youl Kim; Inyoung Kim; Seong Jeong Han; Younghee Ahn; Seung Hyun Cho; Cheol Yong Choi; Kee Oh Chay; Sung Yeul Yang; Bong Whan Ahn; Won Ki Huh; Seung Rock Lee

doi:10.1016/j.febslet.2010.07.006

Redox regulation of the tumor suppressor PTEN by glutathione

Yujeong Kim, Yong Bhum Song, Tae Youl Kim, Inyoung Kim, Seong Jeong Han, Younghee Ahn, Seung Hyun Cho, Cheol Yong Choi, Kee Oh Chay, Sung Yeul Yang, Bong Whan Ahn, Won Ki Huh, Seung Rock Lee

Department of Biological Sciences

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expressed in Saccharomyces cerevisiae was reversibly oxidized by hydrogen peroxide and reduced by cellular reductants. Reduction of hPTEN was delayed in each of S. cerevisiae gsh1Δ and gsh2Δ mutants. Expression of γ-glutamylcysteine synthetase Gsh1 in the gsh1Δ mutant rescued regeneration rate of hPTEN. Oxidized hPTEN was reduced by glutathione in a concentration- and time-dependent manner. Glutathionylated PTEN was detected. Incubation of 293T cells with BSO and knockdown expression of GCLc in HeLa cells by siRNA resulted in the delay of reduction of oxidized PTEN. Also, in HeLa cells transfected with GCLc siRNA, stimulation with epidermal growth factor resulted in the increase of oxidized PTEN and phosphorylation of Akt. These results suggest that the reduction of oxidized hPTEN is mediated by glutathione.

Original language	English
Pages (from-to)	3550-3556
Number of pages	7
Journal	FEBS Letters
Volume	584
Issue number	16
DOIs	https://doi.org/10.1016/j.febslet.2010.07.006
State	Published - Aug 2010

Keywords

Glutathione
Hydrogen peroxide
PTEN
Reactive oxygen species

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10.1016/j.febslet.2010.07.006

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@article{62474779fcdf4ad192f7840181db8ed8,

title = "Redox regulation of the tumor suppressor PTEN by glutathione",

abstract = "Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expressed in Saccharomyces cerevisiae was reversibly oxidized by hydrogen peroxide and reduced by cellular reductants. Reduction of hPTEN was delayed in each of S. cerevisiae gsh1Δ and gsh2Δ mutants. Expression of γ-glutamylcysteine synthetase Gsh1 in the gsh1Δ mutant rescued regeneration rate of hPTEN. Oxidized hPTEN was reduced by glutathione in a concentration- and time-dependent manner. Glutathionylated PTEN was detected. Incubation of 293T cells with BSO and knockdown expression of GCLc in HeLa cells by siRNA resulted in the delay of reduction of oxidized PTEN. Also, in HeLa cells transfected with GCLc siRNA, stimulation with epidermal growth factor resulted in the increase of oxidized PTEN and phosphorylation of Akt. These results suggest that the reduction of oxidized hPTEN is mediated by glutathione.",

keywords = "Glutathione, Hydrogen peroxide, PTEN, Reactive oxygen species",

author = "Yujeong Kim and Song, \{Yong Bhum\} and Kim, \{Tae Youl\} and Inyoung Kim and Han, \{Seong Jeong\} and Younghee Ahn and Cho, \{Seung Hyun\} and Choi, \{Cheol Yong\} and Chay, \{Kee Oh\} and Yang, \{Sung Yeul\} and Ahn, \{Bong Whan\} and Huh, \{Won Ki\} and Lee, \{Seung Rock\}",

year = "2010",

month = aug,

doi = "10.1016/j.febslet.2010.07.006",

language = "English",

volume = "584",

pages = "3550--3556",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc",

number = "16",

}

TY - JOUR

T1 - Redox regulation of the tumor suppressor PTEN by glutathione

AU - Kim, Yujeong

AU - Song, Yong Bhum

AU - Kim, Tae Youl

AU - Kim, Inyoung

AU - Han, Seong Jeong

AU - Ahn, Younghee

AU - Cho, Seung Hyun

AU - Choi, Cheol Yong

AU - Chay, Kee Oh

AU - Yang, Sung Yeul

AU - Ahn, Bong Whan

AU - Huh, Won Ki

AU - Lee, Seung Rock

PY - 2010/8

Y1 - 2010/8

N2 - Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expressed in Saccharomyces cerevisiae was reversibly oxidized by hydrogen peroxide and reduced by cellular reductants. Reduction of hPTEN was delayed in each of S. cerevisiae gsh1Δ and gsh2Δ mutants. Expression of γ-glutamylcysteine synthetase Gsh1 in the gsh1Δ mutant rescued regeneration rate of hPTEN. Oxidized hPTEN was reduced by glutathione in a concentration- and time-dependent manner. Glutathionylated PTEN was detected. Incubation of 293T cells with BSO and knockdown expression of GCLc in HeLa cells by siRNA resulted in the delay of reduction of oxidized PTEN. Also, in HeLa cells transfected with GCLc siRNA, stimulation with epidermal growth factor resulted in the increase of oxidized PTEN and phosphorylation of Akt. These results suggest that the reduction of oxidized hPTEN is mediated by glutathione.

AB - Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expressed in Saccharomyces cerevisiae was reversibly oxidized by hydrogen peroxide and reduced by cellular reductants. Reduction of hPTEN was delayed in each of S. cerevisiae gsh1Δ and gsh2Δ mutants. Expression of γ-glutamylcysteine synthetase Gsh1 in the gsh1Δ mutant rescued regeneration rate of hPTEN. Oxidized hPTEN was reduced by glutathione in a concentration- and time-dependent manner. Glutathionylated PTEN was detected. Incubation of 293T cells with BSO and knockdown expression of GCLc in HeLa cells by siRNA resulted in the delay of reduction of oxidized PTEN. Also, in HeLa cells transfected with GCLc siRNA, stimulation with epidermal growth factor resulted in the increase of oxidized PTEN and phosphorylation of Akt. These results suggest that the reduction of oxidized hPTEN is mediated by glutathione.

KW - Glutathione

KW - Hydrogen peroxide

KW - PTEN

KW - Reactive oxygen species

UR - https://www.scopus.com/pages/publications/77955657057

U2 - 10.1016/j.febslet.2010.07.006

DO - 10.1016/j.febslet.2010.07.006

M3 - Article

C2 - 20637195

AN - SCOPUS:77955657057

SN - 0014-5793

VL - 584

SP - 3550

EP - 3556

JO - FEBS Letters

JF - FEBS Letters

IS - 16

ER -

Redox regulation of the tumor suppressor PTEN by glutathione

Abstract

Keywords

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