TY - JOUR
T1 - Reconstitution of Human Lymphocytes Following Ex Vivo Expansion of Human Umbilical Cord Blood CD34+ Cells and Transplantation in Rag2-/- γc-/- Mice Model
AU - Choi, B. K.
AU - Joo, S. Y.
AU - Moon, C.
AU - Park, K. S.
AU - Kim, S. H.
AU - Park, J. B.
AU - Jung, G. O.
AU - Choi, G. S.
AU - Kwon, C. H.
AU - Chun, J. M.
AU - Joh, J. W.
AU - Lee, S. K.
AU - Kim, S. J.
PY - 2008/10
Y1 - 2008/10
N2 - Background: Due to ethical issues, in vivo studies of the human immune system have been difficult. Thus, small-animal xenotransplantation models have been employed, although they scarcely sustain a human immune response. In this study, we compared human cell repopulation tendencies and functionality in Rag2-/- γc-/- mice following various ex vivo expanded human hematopoietic stem cells (HSCs). Methods: Human umbilical cord blood (UCB) CD34+ cells were cultured for 7 days with a cytokine combination of stem cell factor, Flk2/Flt3 ligand, and thrombopoietin, with absence or presence of rhIL-3, then transplanted into Rag2-/- γc-/- mice. Reconstituted human lymphocytes were analyzed based on the expression of CD45 as well as CD3, CD19, and CD56 in peripheral blood (PB) until 16 weeks after transplantation. BrdU assay and functional analysis of reconstituted human lymphocytes used PHA- or rhIL-2-stimulated splenocytes and bone marrow cells from recipient mice. Results: The percentage of human CD45dim cells, not CD45bright cells, in PB of mice transplanted with cultured HSCs with rhIL-3 was much higher than in the group without rhIL-3 (approximately 2.5-fold at week 10 posttransplantation). The humanized mice showed systemic repopulation with a comprehensive array of human lymphohematopoietic cells, including T, B, natural killer (NK) cells, and even dendritic cells. However, the expression level was also dim. The number of CD3+ T cells and CD56+ NK cells was especially increased in the presence of rhIL-3. In addition, after in vitro restimulation proliferation assays and NK activity of interferon-γ secretion showed greater effects in the presence of rhIL-3. Conclusion: These data suggested that the development of a diverse repopulation of human lymphocytes was possible in Rag2-/-γc-/- mice after transplantation of cultured UCB CD34+ HSCs with interleukin-3.
AB - Background: Due to ethical issues, in vivo studies of the human immune system have been difficult. Thus, small-animal xenotransplantation models have been employed, although they scarcely sustain a human immune response. In this study, we compared human cell repopulation tendencies and functionality in Rag2-/- γc-/- mice following various ex vivo expanded human hematopoietic stem cells (HSCs). Methods: Human umbilical cord blood (UCB) CD34+ cells were cultured for 7 days with a cytokine combination of stem cell factor, Flk2/Flt3 ligand, and thrombopoietin, with absence or presence of rhIL-3, then transplanted into Rag2-/- γc-/- mice. Reconstituted human lymphocytes were analyzed based on the expression of CD45 as well as CD3, CD19, and CD56 in peripheral blood (PB) until 16 weeks after transplantation. BrdU assay and functional analysis of reconstituted human lymphocytes used PHA- or rhIL-2-stimulated splenocytes and bone marrow cells from recipient mice. Results: The percentage of human CD45dim cells, not CD45bright cells, in PB of mice transplanted with cultured HSCs with rhIL-3 was much higher than in the group without rhIL-3 (approximately 2.5-fold at week 10 posttransplantation). The humanized mice showed systemic repopulation with a comprehensive array of human lymphohematopoietic cells, including T, B, natural killer (NK) cells, and even dendritic cells. However, the expression level was also dim. The number of CD3+ T cells and CD56+ NK cells was especially increased in the presence of rhIL-3. In addition, after in vitro restimulation proliferation assays and NK activity of interferon-γ secretion showed greater effects in the presence of rhIL-3. Conclusion: These data suggested that the development of a diverse repopulation of human lymphocytes was possible in Rag2-/-γc-/- mice after transplantation of cultured UCB CD34+ HSCs with interleukin-3.
UR - https://www.scopus.com/pages/publications/53149090166
U2 - 10.1016/j.transproceed.2008.08.011
DO - 10.1016/j.transproceed.2008.08.011
M3 - Article
C2 - 18929829
AN - SCOPUS:53149090166
SN - 0041-1345
VL - 40
SP - 2655
EP - 2660
JO - Transplantation Proceedings
JF - Transplantation Proceedings
IS - 8
ER -
