Rapid and simple purification of a novel extracellular β-amylase from Bacillus sp.

Young Min Ha; Dong Gun Lee; Jung Hoon Yoon; Yong Ha Park; Young Jae Kim

doi:10.1023/A:1011682223796

Rapid and simple purification of a novel extracellular β-amylase from Bacillus sp.

Young Min Ha
, Dong Gun Lee
, Jung Hoon Yoon
, Yong Ha Park
, Young Jae Kim

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Bacillus sp. KYJ 963,a local isolate, produced an extracellular amylase with M_r = 59 kDa. The amylase was easily purified by adsorption on soluble starch. The analyses of TLC and N-terminal amino acid sequence from the purified protein revealed that the enzyme was a novel β-amylase which could not hydrolyze maltose or α-cyclodextrin and its N-terminal amino acid sequence was A-V-N-G-Q-S-F-N-S-N-Y-K-T-Y-K-.

Original language	English
Pages (from-to)	1435-1438
Number of pages	4
Journal	Biotechnology Letters
Volume	23
Issue number	17
DOIs	https://doi.org/10.1023/A:1011682223796
State	Published - 2001
Externally published	Yes

Keywords

Anchovy-jeot
Bacillus sp. KYJ 963
Extracellular β-amylase
Starch adsorption

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10.1023/A:1011682223796

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@article{943053ce5dec4edaaeaf33f93f721ca1,

title = "Rapid and simple purification of a novel extracellular β-amylase from Bacillus sp.",

abstract = "Bacillus sp. KYJ 963,a local isolate, produced an extracellular amylase with Mr = 59 kDa. The amylase was easily purified by adsorption on soluble starch. The analyses of TLC and N-terminal amino acid sequence from the purified protein revealed that the enzyme was a novel β-amylase which could not hydrolyze maltose or α-cyclodextrin and its N-terminal amino acid sequence was A-V-N-G-Q-S-F-N-S-N-Y-K-T-Y-K-.",

keywords = "Anchovy-jeot, Bacillus sp. KYJ 963, Extracellular β-amylase, Starch adsorption",

author = "\{Min Ha\}, Young and \{Gun Lee\}, Dong and Yoon, \{Jung Hoon\} and Park, \{Yong Ha\} and \{Jae Kim\}, Young",

year = "2001",

doi = "10.1023/A:1011682223796",

language = "English",

volume = "23",

pages = "1435--1438",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer Science and Business Media B.V.",

number = "17",

}

TY - JOUR

T1 - Rapid and simple purification of a novel extracellular β-amylase from Bacillus sp.

AU - Min Ha, Young

AU - Gun Lee, Dong

AU - Yoon, Jung Hoon

AU - Park, Yong Ha

AU - Jae Kim, Young

PY - 2001

Y1 - 2001

N2 - Bacillus sp. KYJ 963,a local isolate, produced an extracellular amylase with Mr = 59 kDa. The amylase was easily purified by adsorption on soluble starch. The analyses of TLC and N-terminal amino acid sequence from the purified protein revealed that the enzyme was a novel β-amylase which could not hydrolyze maltose or α-cyclodextrin and its N-terminal amino acid sequence was A-V-N-G-Q-S-F-N-S-N-Y-K-T-Y-K-.

AB - Bacillus sp. KYJ 963,a local isolate, produced an extracellular amylase with Mr = 59 kDa. The amylase was easily purified by adsorption on soluble starch. The analyses of TLC and N-terminal amino acid sequence from the purified protein revealed that the enzyme was a novel β-amylase which could not hydrolyze maltose or α-cyclodextrin and its N-terminal amino acid sequence was A-V-N-G-Q-S-F-N-S-N-Y-K-T-Y-K-.

KW - Anchovy-jeot

KW - Bacillus sp. KYJ 963

KW - Extracellular β-amylase

KW - Starch adsorption

UR - https://www.scopus.com/pages/publications/0034813040

U2 - 10.1023/A:1011682223796

DO - 10.1023/A:1011682223796

M3 - Article

AN - SCOPUS:0034813040

SN - 0141-5492

VL - 23

SP - 1435

EP - 1438

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 17

ER -

Rapid and simple purification of a novel extracellular β-amylase from Bacillus sp.

Abstract

Keywords

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