TY - JOUR
T1 - Quantitative Serodiagnosis of Scrub Typhus Using Surface-Enhanced Raman Scattering-Based Lateral Flow Assay Platforms
AU - Lee, See Hi
AU - Hwang, Joonki
AU - Kim, Kihyun
AU - Jeon, Jinhyeok
AU - Lee, Sangyeop
AU - Ko, Juhui
AU - Lee, Jichul
AU - Kang, Minhee
AU - Chung, Doo Ryeon
AU - Choo, Jaebum
N1 - Publisher Copyright:
Copyright © 2019 American Chemical Society.
PY - 2019/10/1
Y1 - 2019/10/1
N2 - A surface-enhanced Raman scattering-based lateral flow assay (SERS-LFA) technique has been developed for the rapid and accurate diagnosis of scrub typhus. Lateral flow kits for the detection of O. tsutsugamushi IgG (scrub typhus biomarker) were fabricated, and the calibration curve for various standard clinical sera concentrations were obtained by Raman measurements. The clinical sera titer values were determined by fitting the Raman data to the calibration curve. To assess the clinical feasibility of the proposed method, SERS-LFA assays were performed on 40 clinical samples. The results showed good agreement with those of the standard indirect immunofluorescence assay (IFA) method. SERS-LFA has many advantages over IFA including the less sample volume, simpler assay steps, shorter assay time, more systematic quantitative analysis, and longer assay lifetime. As SERS strips can be easily integrated with a miniaturized Raman spectrophotometer, field serodiagnosis is also more feasible.
AB - A surface-enhanced Raman scattering-based lateral flow assay (SERS-LFA) technique has been developed for the rapid and accurate diagnosis of scrub typhus. Lateral flow kits for the detection of O. tsutsugamushi IgG (scrub typhus biomarker) were fabricated, and the calibration curve for various standard clinical sera concentrations were obtained by Raman measurements. The clinical sera titer values were determined by fitting the Raman data to the calibration curve. To assess the clinical feasibility of the proposed method, SERS-LFA assays were performed on 40 clinical samples. The results showed good agreement with those of the standard indirect immunofluorescence assay (IFA) method. SERS-LFA has many advantages over IFA including the less sample volume, simpler assay steps, shorter assay time, more systematic quantitative analysis, and longer assay lifetime. As SERS strips can be easily integrated with a miniaturized Raman spectrophotometer, field serodiagnosis is also more feasible.
UR - https://www.scopus.com/pages/publications/85071728434
U2 - 10.1021/acs.analchem.9b02363
DO - 10.1021/acs.analchem.9b02363
M3 - Article
C2 - 31356055
AN - SCOPUS:85071728434
SN - 0003-2700
VL - 91
SP - 12275
EP - 12282
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 19
ER -