Pseudoruegeria sabulilitoris sp. nov., Isolated from seashore sand

A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated and ovoid or rod-shaped bacterial strain, GJMS-35^T, was isolated from seashore sand at Geoje Island, South Korea. Strain GJMS-35^T grew optimally at 28-30 °C, at pH 7.0-8.0 and in the presence of 2.0% (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain GJMS-35^T clustered with type strains of species of the genus Pseudoruegeria, with which it exhibited 96.97-98.42% 16S rRNA gene sequence similarity. Sequence similarities to the type strains of other recognized species were less than 96.39 %. Strain GJMS-35^T contained Q-10 as the predominant ubiquinone and C₁₈ : 1ω7c as the major fatty acid. The major polar lipids of strain GJMS-35^T were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified glycolipid, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain GJMS-35^T was 64.1 mol% and its mean DNA-DNA relatedness values with type strains of three species of the genus Pseudoruegeria were 11-21 %. Its differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain GJMS-35^T is set apart from other species of the genus Pseudoruegeria. On the basis of the data presented, strain GJMS-35^T is considered to represent a novel species of the genus Pseudoruegeria, for which the name Pseudoruegeria sabulilitoris sp. nov. is proposed. The type strain is GJMS-35^T (=KCTC 42111^T=NBRC 110380^T).