Pseudoruegeria insulae sp. nov., isolated from a tidal flat

A Gram-stain-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, designated BPTF-M20 ^T , was isolated from tidal flat sediment in the Yellow Sea, Republic of Korea. Strain BPTF-M20 ^T grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0-3.0% (w/v) NaCl. A neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain BPTF-M20 ^T fell within the clade comprising the type strains of Pseudoruegeria species. Strain BPTF-M20 ^T exhibited 16S rRNA gene sequence similarity values of 97.4-98.3% to the type strains of Pseudoruegeria haliotis, Pseudoruegeria lutimaris, ‘Pseudoruegeria litorisediminis’ and Pseudoruegeria sabulilitoris and 96.4-96.9% to the type strains of the other Pseudoruegeria species. Strain BPTF-M20 ^T contained Q-10 as the predominant ubiquinone and C _{18: 1ω7c} as the major fatty acid. The major polar lipids detected in strain BPTF-M20 ^T were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminolipid and one unidentified glycolipid. The DNA G+C content of strain BPTF-M20 ^T was 63.2 mol%. Mean DNA-DNA relatedness values of strain BPTF-M20 ^T with the type strains of P. haliotis, P. lutimaris, P. sabulilitoris and ‘P. litorisediminis’ were 18-27 %. Differential phenotypic properties, together with the phylogenetic and genetic data, revealed that strain BPTF-M20 ^T was separated from recognized Pseudoruegeria species. On the basis of the data presented here, strain BPTF-M20 ^T is considered to represent a novel species of the genus Pseudoruegeria, for which the name Pseudoruegeria insulae sp. nov. is proposed. The type strain is BPTF-M20 ^T (=KACC 19614 ^T =KCTC 62422 ^T =NBRC 113188 ^T ).