Pseudorhodobacter wandonensis sp. nov., isolated from wood falls, and emended description of the genus Pseudorhodobacter

A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterial strain, designated WT-MW11^T, was isolated from wood falls on the coast of Wando, an island of South Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain WT-MW11^T grew optimally at pH 7.0-8.0, at 25 °C and in the presence of 2.0-3.0 % (w/v) NaCl. Bacteriochlorophyll a was not produced. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain WT-MW11^T clustered with Pseudorhodobacter ferrugineus IAM 12616^T and Pseudorhodobacter aquimaris HDW-19^T, with which it exhibited 16S rRNA gene sequence similarity values of 97.9 and 95.5%, respectively. Strain WT-MW11^T contained Q-10 as the predominant ubiquinone and C_18:1 ω7c as the major fatty acid. The polar lipid profile of strain WT-MW11^T was similar to those of P. ferrugineus JCM 20687^T and P. aquimaris HDW-19^T. The DNA G+C content of strain WT-MW11^T was 61.6 mol% and its mean DNA-DNA relatedness value with P. ferrugineus JCM 20687^T was 12.3%. The phylogenetic and genetic distinctiveness and differential phenotypic properties revealed that strain WT-MW11^T was distinguishable from the two recognized species of the genus Pseudorhodobacter. On the basis of the data presented, strain WT-MW11^T is considered to represent a novel species of the genus Pseudorhodobacter, for which the name Pseudorhodobacter wandonensis sp. nov. is proposed. The type strain is WT-MW11^T (=KCTC 23672^T=CCUG 61506^T). The description of the genus Pseudorhodobacter is emended.