Pseudomaribius aestuariivivens gen. Nov., sp. nov., isolated from a tidal flat sediment

A Gram-stain-negative, non-motile and ovoid or rod-shaped bacterial strain, DBTF-15T, was isolated from a tidal flat sediment on the Yellow Sea in South Korea. Strain DBTF-15^T grew optimally at 25–30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences revealed that strain DBTF-15^T joined the cluster comprising the type strains of species of the genus Palleronia. Strain DBTF-15^T exhibited higher 16S rRNA gene sequence similarity values to the type strains (96.5–96.7 %) of Maribius pelagius and Maribius salinus than to those (94.6–96.1 %) of the three species of the genus Palleronia. It exhibited 16S rRNA gene sequence similarity values of less than 93.9 % to the type strains of the other recognized species. Strain DBTF-15^T contained Q-10 as the predominant ubiquinone and C_18 : 1 ω7c as the major fatty acid. The major polar lipids of strain DBTF-15^T were phosphatidylcholine, phosphatidylglycerol and one unidentified aminolipid. The DNA G+C content of strain DBTF-15^T was 68.7 mol%. The chemotaxonomic data and other differential phenotypic properties made it possible to distinguish strain DBTF-15^T from the genera Maribius and Palleronia. On the basis of the data presented, strain DBTF-15^T constitutes a novel genus and species within the class Alphaproteobacteria, for which the name Pseudomaribius aestuariivivens gen. nov., sp. nov. is proposed. The type strain is DBTF-15^T (=KACC 19431^T=NBRC 113039^T).