Pseudohalocynthiibacter aestuariivivens gen. nov., sp. nov., isolated from a tidal flat

A Gram-stain-negative, non-motile, aerobic and coccoid, ovoid or rod-shaped bacterial strain, designated BS-W9^T , was isolated from a tidal flat of the South Sea, South Korea. Strain BS-W9^T grew optimally at 25–30 6C, at pH 7.0–8.0 and in the presence of approximately 2.0 % (w/v) NaCl. Phylogenetic trees, based on 16S rRNA gene sequences, revealed that strain BS-W9^T clustered with the type strain of Halocynthiibacter namhaensis, showing a highest sequence similarity of 97.3 %. It exhibited sequence similarity values of less than 95.6 % to the type strains of other species with validly published names. Strain BS-W9^T contained Q-10 as the predominant ubiquinone and C_{18 : 1} v7c as the predominant fatty acid. The major polar lipids of strain BS-W9^T were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unidentified lipid and one unidentified aminolipid. The fatty acid and polar lipid profiles of strain BS-W9^T were distinguished from those of the type strains of H. namhaensis and other phylogenetically related genera. The DNA G+C content of strain BS-W9^T was 53.2 mol% and its mean DNA–DNA relatedness value with H. namhaensis RA2-3^T was 14 %. On the basis of the phylogenetic, chemotaxonomic and other phenotypic properties, strain BS-W9^T is considered to represent a novel genus and species within the family Rhodobacteraceae, for which the name Pseudohalocyntiibacter aestuariivivens gen. nov., sp. nov. is proposed. The type strain of Pseudohalocyntiibacter aestuariivivens is BS-W9^T (5KCTC 42348^T 5CECT 8726^T).