Pseudoalteromonas aestuariivivens sp. Nov., isolated from a tidal flat

A Gram-stain-negative, aerobic, motile, rod-shaped or ovoid bacterial strain, designated DB-2^T, was isolated from a tidal flat of the Yellow Sea in South Korea, and subjected to a taxonomic study using a polyphasic approach. Strain DB-2^T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain DB-2^T belonged to the genus Pseudoalteromonas. Strain DB-2^T exhibited 16S rRNA gene sequence similarities of 97.17–97.36 % to the type strains of Pseudoalteromonas mariniglutinosa, Pseudoalteromonas spongiae and Pseudoalteromonas tetraodonis and of 93.79–96.99 % to the type strains of the other species of the genus Pseudoalteromonas. Strain DB-2^T contained Q-8 as the predominant ubiquinone and C_{16: 0}, C_{18: 1}ω7c, summed feature 3 (C_{16: 1}ω7c and/or C_{16: 1}ω6c) and C_{12: 0} 3-OH as the major fatty acids. The major polar lipids detected in strain DB-2^T were phosphatidylglycerol, phosphatidylethanolamine, two unidentified glycolipids, an unidentified phospholipid and an unidentified aminolipid. The DNA G+C content of strain DB-2^T was 54.9±0.2 mol% and mean DNA–DNA relatedness values with the type strains of P. mariniglutinosa, P. spongiae and P. tetraodonis were 10–17 %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain DB-2^T is separated from recognized species of the genus Pseudoalteromonas. On the basis of these data, strain DB-2^T is considered to represent a novel species of the genus Pseudoalteromonas, for which the name Pseudoalteromonas aestuariivivens sp. nov. is proposed. The type strain is DB-2^T (=KCTC 42779^T=CECT 8945^T).