Abstract
Heme oxygenase-1 (HO-1) catalyses the ratelimiting step of heme degradation to biliverdin, which is in turn reduced to bilirubin, CO and free iron. HO-1 can be induced by several harmful stimuli including oxidative stress, and it has a protective role against the cytotoxicity in different cells. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridinium (MPP+) is a neurotoxic substance that induces the degeneration of dopaminergic neurons. This study examined whether HO-1 can be induced by MPP+ and whether HO-1 has a protective role against the MPP+-induced cytotoxicity in PC-12 cells.MPP+ triggered a relatively rapid induction of HO-1. The MPP+-induced cytotoxicity and reactive oxygen species (ROS) production markedly increased by HO-1 inhibitor, zinc protoporphyrin-IX(ZnPP-IX) . The increase of ROS production by ZnPP-IX was completely abrogated by either two products of HO (biliverdin or bilirubin) while the increase of cytotoxicity by ZnPP-IX was attenuated partially.These suggest that HO-1 expression might have some cytoprotective effect against MPP+-induced cytotoxicity.
| Original language | English |
|---|---|
| Pages (from-to) | 307-313 |
| Number of pages | 7 |
| Journal | Neurological Sciences |
| Volume | 31 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jun 2010 |
Keywords
- 1-Methyl-4-phenyl-1,2,3,6- tetrahydropyridinium (MPP)
- Cytotoxicity
- Heme oxygenase-1 (HO-1)
- PC-12 cell
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