Prospective identification of cancer stem cells with the surface antigen CD133.

Kyeung Min Joo; Do Hyun Nam

doi:10.1007/978-1-59745-280-9_5

Prospective identification of cancer stem cells with the surface antigen CD133.

Kyeung Min Joo, Do Hyun Nam

Sungkyunkwan University

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Cancer cells do not share equal tumor-initiating potential. Only cancer stem cells (CSCs) can initiate cancer, which is important clinically because they should be eradicated to treat cancer patients. The purpose of experimental methods for identification of CSC is to isolate CSCs among various kinds of cancer cells in cancer masses. To identify CSCs, cancer masses derived from patients should be dissociated into single cells. Dissociated cells are classified into several groups according to expression status of one or several surface proteins using magnetic cell sorting (MACS) or fluorescence-activated cell sorting (FACS) methods. Sorted cells are cultured in a specialized culture medium for stem cells or inoculated into the primary cancer site of immunodeficient mice. In this chapter detailed experimental methods will be described and glioblastoma will be used as an example of solid cancers.

Original language	English
Pages (from-to)	57-71
Number of pages	15
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	568
DOIs	https://doi.org/10.1007/978-1-59745-280-9_5
State	Published - 2009
Externally published	Yes

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10.1007/978-1-59745-280-9_5

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title = "Prospective identification of cancer stem cells with the surface antigen CD133.",

abstract = "Cancer cells do not share equal tumor-initiating potential. Only cancer stem cells (CSCs) can initiate cancer, which is important clinically because they should be eradicated to treat cancer patients. The purpose of experimental methods for identification of CSC is to isolate CSCs among various kinds of cancer cells in cancer masses. To identify CSCs, cancer masses derived from patients should be dissociated into single cells. Dissociated cells are classified into several groups according to expression status of one or several surface proteins using magnetic cell sorting (MACS) or fluorescence-activated cell sorting (FACS) methods. Sorted cells are cultured in a specialized culture medium for stem cells or inoculated into the primary cancer site of immunodeficient mice. In this chapter detailed experimental methods will be described and glioblastoma will be used as an example of solid cancers.",

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AB - Cancer cells do not share equal tumor-initiating potential. Only cancer stem cells (CSCs) can initiate cancer, which is important clinically because they should be eradicated to treat cancer patients. The purpose of experimental methods for identification of CSC is to isolate CSCs among various kinds of cancer cells in cancer masses. To identify CSCs, cancer masses derived from patients should be dissociated into single cells. Dissociated cells are classified into several groups according to expression status of one or several surface proteins using magnetic cell sorting (MACS) or fluorescence-activated cell sorting (FACS) methods. Sorted cells are cultured in a specialized culture medium for stem cells or inoculated into the primary cancer site of immunodeficient mice. In this chapter detailed experimental methods will be described and glioblastoma will be used as an example of solid cancers.

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SN - 1064-3745

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JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

Prospective identification of cancer stem cells with the surface antigen CD133.

Abstract

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