@inbook{762b24782674489c94870f619f42eabc,
title = "Profiling genome-wide specificity of CRISPR-Cas9 using digenome-seq",
abstract = "Digenome-seq is a highly sensitive method for analyzing the genome-wide specificity of CRISPR-Cas9 nuclease activity. In this procedure, genomic DNA is first subjected to digestion by CRISPR-Cas9 in vitro and then to whole genome sequencing, which results in unusual patterns of straight alignments at on-target and potential off-target sites. Analysis of these data with the Digenome-seq computer program allows for identification of the in vitro cleavage sites associated with the straight alignments. Here, we present a detailed Digenome-seq protocol for genome-wide profiling of in vitro CRISPR-Cas9 nuclease cleavage sites.",
keywords = "Cas9, CRISPR, Genome editing, Off-target, Whole-genome sequencing",
author = "Daesik Kim and Kim, \{Jin Soo\}",
note = "Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-0687-2\_13",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "233--242",
booktitle = "Methods in Molecular Biology",
}