Production and characterization of methyl ester sophorolipids with 22-carbon-fatty acids

Jae Dong Shin; Jeongmi Lee; Yong Bum Kim; In sun Han; Eun Ki Kim

doi:10.1016/j.biortech.2009.12.019

Production and characterization of methyl ester sophorolipids with 22-carbon-fatty acids

Jae Dong Shin
, Jeongmi Lee
, Yong Bum Kim
, In sun Han
, Eun Ki Kim

Department of Pharmacy

National Research Lab.
KCI Ltd.

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

In this study, various fatty acids of 18 and 22 carbons were used as substrates to produce sophorolipids in Candida bombicola ATCC 22214. Methyl esterification of erucic acid and C22-enriched rapeseed oil generated mainly acidic sophorolipids with different degrees of saturation in the fatty acid chain, which was demonstrated by structural analysis using HPLC, LC-MS and NMR spectroscopy. Regardless of methyl esterification, the rapeseed oil served as the best substrate for high production of sophorolipids among the tested. Methyl esterification also had no noticeable effect on the interfacial properties of sophorolipids. However, 22 carbons introduced in the fatty acid chain increased the hydrophobicity of sophorolipids, and therefore improved surface-active properties and biodegradability.

Original language	English
Pages (from-to)	3170-3174
Number of pages	5
Journal	Bioresource Technology
Volume	101
Issue number	9
DOIs	https://doi.org/10.1016/j.biortech.2009.12.019
State	Published - May 2010

Keywords

C22-enriched rapeseed oil
Candida bombicola ATCC 22214
Methyl ester sophorolipids

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10.1016/j.biortech.2009.12.019

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title = "Production and characterization of methyl ester sophorolipids with 22-carbon-fatty acids",

abstract = "In this study, various fatty acids of 18 and 22 carbons were used as substrates to produce sophorolipids in Candida bombicola ATCC 22214. Methyl esterification of erucic acid and C22-enriched rapeseed oil generated mainly acidic sophorolipids with different degrees of saturation in the fatty acid chain, which was demonstrated by structural analysis using HPLC, LC-MS and NMR spectroscopy. Regardless of methyl esterification, the rapeseed oil served as the best substrate for high production of sophorolipids among the tested. Methyl esterification also had no noticeable effect on the interfacial properties of sophorolipids. However, 22 carbons introduced in the fatty acid chain increased the hydrophobicity of sophorolipids, and therefore improved surface-active properties and biodegradability.",

keywords = "C22-enriched rapeseed oil, Candida bombicola ATCC 22214, Methyl ester sophorolipids",

author = "Shin, \{Jae Dong\} and Jeongmi Lee and Kim, \{Yong Bum\} and Han, \{In sun\} and Kim, \{Eun Ki\}",

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doi = "10.1016/j.biortech.2009.12.019",

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volume = "101",

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TY - JOUR

T1 - Production and characterization of methyl ester sophorolipids with 22-carbon-fatty acids

AU - Shin, Jae Dong

AU - Lee, Jeongmi

AU - Kim, Yong Bum

AU - Han, In sun

AU - Kim, Eun Ki

PY - 2010/5

Y1 - 2010/5

N2 - In this study, various fatty acids of 18 and 22 carbons were used as substrates to produce sophorolipids in Candida bombicola ATCC 22214. Methyl esterification of erucic acid and C22-enriched rapeseed oil generated mainly acidic sophorolipids with different degrees of saturation in the fatty acid chain, which was demonstrated by structural analysis using HPLC, LC-MS and NMR spectroscopy. Regardless of methyl esterification, the rapeseed oil served as the best substrate for high production of sophorolipids among the tested. Methyl esterification also had no noticeable effect on the interfacial properties of sophorolipids. However, 22 carbons introduced in the fatty acid chain increased the hydrophobicity of sophorolipids, and therefore improved surface-active properties and biodegradability.

AB - In this study, various fatty acids of 18 and 22 carbons were used as substrates to produce sophorolipids in Candida bombicola ATCC 22214. Methyl esterification of erucic acid and C22-enriched rapeseed oil generated mainly acidic sophorolipids with different degrees of saturation in the fatty acid chain, which was demonstrated by structural analysis using HPLC, LC-MS and NMR spectroscopy. Regardless of methyl esterification, the rapeseed oil served as the best substrate for high production of sophorolipids among the tested. Methyl esterification also had no noticeable effect on the interfacial properties of sophorolipids. However, 22 carbons introduced in the fatty acid chain increased the hydrophobicity of sophorolipids, and therefore improved surface-active properties and biodegradability.

KW - C22-enriched rapeseed oil

KW - Candida bombicola ATCC 22214

KW - Methyl ester sophorolipids

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U2 - 10.1016/j.biortech.2009.12.019

DO - 10.1016/j.biortech.2009.12.019

M3 - Article

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AN - SCOPUS:74649085231

SN - 0960-8524

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SP - 3170

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JO - Bioresource Technology

JF - Bioresource Technology

IS - 9

ER -

Production and characterization of methyl ester sophorolipids with 22-carbon-fatty acids

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Keywords

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