Polaribacter litorisediminis sp. Nov., isolated from a tidal flat

A Gram-stain-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, designated OITF-11^T, was isolated from a tidal flat in Oido, an island of South Korea, and subjected to a polyphasic taxonomic study. Strain OITF-11^T grew optimally at 25 ºC, at pH 7.0–8.0 and in the presence of 2.0% (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that strain OITF-11T belonged to the genus Polaribacter. Strain OITF-11^T exhibited 16S rRNA gene sequence similarity values of 97.4–98.1% to Polaribacter haliotis RA4-7^T, Polaribacter atrinae KACC 17473^T, Polaribacter dokdonensis DSW-5T and Polaribacter marinaquae KCTC 42664^T, and of 94.1–96.9% to the type strains of the other Polaribacter species. Strain OITF-11^T contained menaquinone MK-6 as the predominant menaquinone and iso-C_{15 : 0}, iso-C_{15 : 1} G, iso-C_{15 : 0} 3-OH, iso-C_{17 : 0} 3-OH and C_{15 : 1}ω6c as the major fatty acids. The major polar lipids detected in strain OITF-11^T were phosphatidylethanolamine, phosphatidylmonomethylethanolamine and one unidentified lipid. The DNA G+C content of strain OITF-11T was 32.2 mol% and its DNA–DNA relatedness values with the type strains of P. haliotis, P. atrinae, P. dokdonensis and P. marinaquae were 14–33 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain OITF-11T is separated from recognized species of the genus Polaribacter. On the basis of the data presented, strain OITF-11^T is considered to represent a novel species of the genus Polaribacter, for which the name Polaribacter litorisediminis sp. nov. is proposed. The type strain is OITF-11^T (=KCTC 52500^T=NBRC 112457^T).