Paraperlucidibaca wandonensis sp. nov., isolated from seawater, and emended description of the genus Paraperlucidibaca Oh et al. 2011

A Gram-stain-negative, aerobic, motile and rod-shaped bacterial strain, designated WT-RY4^T, was isolated from wood falls in the South Sea, South Korea, and its taxonomic position was investigated using a polyphasic approach. Strain WT-RY4^T grew optimally at 25 °C, at pH 7.0-7.5 and in the absence of NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain WT-RY4^T clustered with the type strain of Paraperlucidibaca baekdonensis with a bootstrap resampling value of 100%. Strain WT-RY4^T exhibited 16S rRNA gene sequence similarity values of 98.8% and 96.3% to Paraperlucidibaca baekdonensis RL-2^T and Perlucidibaca piscinae IMCC 1704^T, respectively and less than 91.5% to the type strains of other species used in the phylogenetic analysis. The DNA G+C content of strain WT-RY4^T was 52.4 mol% and the mean DNA-DNA relatedness value with Paraperlucidibaca baekdonensis RL-2^T was 25%. Strain WT-RY4^T contained Q-11 as the predominant ubiquinone and summed feature 3 (C_16:1v7c and/or C_16:1v6c), C_16:0 and C_12:0 3-OH as the major fatty acids. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, demonstrated that strain WT-RY4^T was distinguishable from Paraperlucidibaca baekdonensis RL-2^T. On the basis of the data presented, strain WT-RY4^T is considered to represent a novel species of the genus Paraperlucidibaca, for which the name Paraperlucidibaca wandonensis sp. nov. is proposed. The type strain is WT-RY4^T (=KCTC 32216^T=CCUG 63419^T). An emended description of the genus Paraperlucidibaca is also provided.