Paraglaciecola aestuariivivens sp. Nov., isolated from a tidal flat

A Gram-stain-negative, singly flagellated, aerobic and coccoid, ovoid or rod-shaped bacterium, designated strain JDTF-33^T, was isolated from a tidal flat in Jindo, an island of South Korea. Strain JDTF-33^T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0% (w/v) NaCl. A neighbour-joining phylogenetic tree, based on 16S rRNA gene sequences, demonstrated that strain JDTF-33^T belonged to the genus Paraglaciecola, joining the type strain of the species Paraglaciecola aquimarina with 97.9% sequence similarity. Strain JDTF-33^T exhibited 16S rRNA gene sequence similarities of 97.1 and 97.0% to the type strains of Paraglaciecola arctica and Paraglaciecola psychrophila, respectively, and of 96.1–96.6% to the type strains of the other species of the genus Paraglaciecola. Strain JDTF-33^T showed DNA–DNA relatedness values of 11– 24% to the type strains of the species P. aquimarina, P. arctica and Paraglaciecola psychrophila. Strain JDTF-33^T contained Q-8 as the predominant ubiquinone and summed feature 3 (C_16:ω7c and/or C_{16: 1}ω6c) and C_{16: 0} as the major fatty acids. The major polar lipids detected in strain JDTF-33T were phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content of strain JDTF-33^T was 41.7 mol%. Differential phenotypic properties, together with phylogenetic and genetic data, demonstrate that strain JDTF-33^T is separate from species of the genus Paraglaciecola with validly published names. On the basis of the data presented, strain JDTF-33^T represents a novel species of the genus Paraglaciecola, for which the name Paraglaciecola aestuariivivens sp. nov. is proposed. The type strain is JDTF-33^T (=KCTC 52838^T=NBRC 112782^T).