Paenimaribius caenipelagi gen. Nov., sp. nov., isolated from a tidal flat

A Gram-stain-negative, strictly aerobic, non-motile and ovoid- or rod-shaped bacterial strain, JBTF-M29^T, was isolated from tidal flat sediment sampled from the Yellow Sea, Republic of Korea. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain JBTF-M29^T clustered with the type strains of Maribius species and this cluster joined the clade comprising Pseudomaribius and Palleronia species. Strain JBTF-M29^T exhibited 16S rRNA gene sequence similarity values of 96.2–96.6 % to the type strains of three Maribius species, of 96.4 % to the type strain of Pseudomaribius aestuariivivens and of 93.9–94.7 % to the type strains of three Palleronia species. In the UPGMA dendrogram based on the ANI values of genomic sequences, strain JBTF-M29^T formed an evolutionary lineage independent of the genera Maribius and Palleronia and some other taxa. Strain JBTF-M29^T contained Q-10 as the predominant ubiquinone and C_18 : 1  ω7c and C_18 : 0 as the major fatty acids. The major polar lipids of strain JBTF-M29^T were phosphatidylglycerol and one unidentified aminolipid. The DNA G+C content of strain JBTF-M29^T was 64.5 mol%. The differences in fatty acid and polar lipid profiles and other differential phenotypic properties including nitrate reduction and casein hydrolysis made it reasonable to distinguish strain JBTF-M29^T from the genera Maribius, Pseudomaribius and Palleronia . Therefore, on the basis of the polyphasic taxonomic data presented here, strain JBTF-M29^T constitutes a new genus and species within the class Alphaproteobacteria, for which the name Paenimaribius caenipelagi gen. nov., sp. nov. is proposed. The type strain is JBTF-M29^T (=KACC 19867^T=NBRC 113548^T).