Optimal conditions for the retrieval of CD4 and CD8 antigens in formalin-fixed, paraffin-embedded tissues

Seok H. Kim; Myeong C. Kook; Hyung G. Song

doi:10.1023/B:HIJO.0000039856.43632.66

Optimal conditions for the retrieval of CD4 and CD8 antigens in formalin-fixed, paraffin-embedded tissues

Seok H. Kim
, Myeong C. Kook
, Hyung G. Song

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The effects of buffer, NaCl, EDTA, and urea on the retrieval of CD4 and CD8 antigens in formaldehyde-fixed, paraffin-embedded tissues with a microwave pressure-cooker were evaluated. The optimal retrieval conditions were found to be borate buffer at pH 8 containing 1 mM NaCl and 1 mM EDTA. Urea was found to be less effective.

Original language	English
Pages (from-to)	403-408
Number of pages	6
Journal	Journal of Molecular Histology
Volume	35
Issue number	4
DOIs	https://doi.org/10.1023/B:HIJO.0000039856.43632.66
State	Published - 2004
Externally published	Yes

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10.1023/B:HIJO.0000039856.43632.66

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title = "Optimal conditions for the retrieval of CD4 and CD8 antigens in formalin-fixed, paraffin-embedded tissues",

abstract = "The effects of buffer, NaCl, EDTA, and urea on the retrieval of CD4 and CD8 antigens in formaldehyde-fixed, paraffin-embedded tissues with a microwave pressure-cooker were evaluated. The optimal retrieval conditions were found to be borate buffer at pH 8 containing 1 mM NaCl and 1 mM EDTA. Urea was found to be less effective.",

author = "Kim, \{Seok H.\} and Kook, \{Myeong C.\} and Song, \{Hyung G.\}",

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AU - Kook, Myeong C.

AU - Song, Hyung G.

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Y1 - 2004

N2 - The effects of buffer, NaCl, EDTA, and urea on the retrieval of CD4 and CD8 antigens in formaldehyde-fixed, paraffin-embedded tissues with a microwave pressure-cooker were evaluated. The optimal retrieval conditions were found to be borate buffer at pH 8 containing 1 mM NaCl and 1 mM EDTA. Urea was found to be less effective.

AB - The effects of buffer, NaCl, EDTA, and urea on the retrieval of CD4 and CD8 antigens in formaldehyde-fixed, paraffin-embedded tissues with a microwave pressure-cooker were evaluated. The optimal retrieval conditions were found to be borate buffer at pH 8 containing 1 mM NaCl and 1 mM EDTA. Urea was found to be less effective.

UR - https://www.scopus.com/pages/publications/16644401710

U2 - 10.1023/B:HIJO.0000039856.43632.66

DO - 10.1023/B:HIJO.0000039856.43632.66

M3 - Article

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AN - SCOPUS:16644401710

SN - 1567-2379

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SP - 403

EP - 408

JO - Journal of Molecular Histology

JF - Journal of Molecular Histology

IS - 4

ER -

Optimal conditions for the retrieval of CD4 and CD8 antigens in formalin-fixed, paraffin-embedded tissues

Abstract

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