TY - JOUR
T1 - One-step construction of a molybdenum disulfide/multi-walled carbon nanotubes/polypyrrole nanocomposite biosensor for the ex-vivo detection of dopamine in mouse brain tissue
AU - Vijayaraj, Kathiresan
AU - Dinakaran, Thirumalai
AU - Lee, Yujeong
AU - Kim, Suhkmann
AU - Kim, Hyung Sik
AU - Lee, Jaewon
AU - Chang, Seung Cheol
N1 - Publisher Copyright:
© 2017 Elsevier Inc.
PY - 2017/12/9
Y1 - 2017/12/9
N2 - We developed a new strategy for construction of a biosensor for the neurotransmitter dopamine. The biosensor was constructed by one-step electrochemical deposition of a nanocomposite in aqueous solution at pH 7.0, consisting of molybdenum disulfide, multi-walled carbon nanotubes, and polypyrrole. A series of analytical methods was performed to investigate the surface characteristics and the improved electrocatalytic effect of the nanocomposite, including cyclic voltammetry, electrochemical impedance spectroscopy, field-emission scanning electron microscopy, atomic force microscopy, and Raman spectroscopy. The constructed biosensor showed high sensitivity (1.130 μAμM−1cm−2) with a dynamic linearity range of 25–1000 nM and a detection limit of 10 nM. Additionally, the designed sensor exhibited strong anti-interference ability and satisfactory reproducibility. The practical application of the sensor was manifested for the ex vivo determination of dopamine neurotransmitters using brain tissue samples of a mouse Parkinson's disease model.
AB - We developed a new strategy for construction of a biosensor for the neurotransmitter dopamine. The biosensor was constructed by one-step electrochemical deposition of a nanocomposite in aqueous solution at pH 7.0, consisting of molybdenum disulfide, multi-walled carbon nanotubes, and polypyrrole. A series of analytical methods was performed to investigate the surface characteristics and the improved electrocatalytic effect of the nanocomposite, including cyclic voltammetry, electrochemical impedance spectroscopy, field-emission scanning electron microscopy, atomic force microscopy, and Raman spectroscopy. The constructed biosensor showed high sensitivity (1.130 μAμM−1cm−2) with a dynamic linearity range of 25–1000 nM and a detection limit of 10 nM. Additionally, the designed sensor exhibited strong anti-interference ability and satisfactory reproducibility. The practical application of the sensor was manifested for the ex vivo determination of dopamine neurotransmitters using brain tissue samples of a mouse Parkinson's disease model.
KW - Dopamine
KW - Ex vivo sensor
KW - Molybdenum disulfide
KW - Mouse Parkinson's disease model
KW - One-step electrochemical deposition
UR - https://www.scopus.com/pages/publications/85032427824
U2 - 10.1016/j.bbrc.2017.10.059
DO - 10.1016/j.bbrc.2017.10.059
M3 - Article
C2 - 29037811
AN - SCOPUS:85032427824
SN - 0006-291X
VL - 494
SP - 181
EP - 187
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1-2
ER -