Octadecabacter ascidiaceicola sp. nov., isolated from a sea squirt (Halocynthia roretzi)

A Gram-stain-negative, non-spore-forming, non-flagellated and coccoid, ovoid or rod-shaped bacterial strain, RA1-3^T, was isolated from a sea squirt (Halocynthia roretzi) collected from the South Sea, South Korea, and subjected to a taxonomic study using a polyphasic approach. Strain RA1-3^Tgrew optimally at 25 8C, at pH 7.0–8.0 and in the presence of 2.0–3.0% (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain RA1-3^Tclustered with the type strains of three species of the genus Octadecabacter, showing 97.54–98.41% 16S rRNA gene sequence similarity. Sequence similarities to other recognized species were less than 96.97%. Strain RA1-3^Tcontained Q-10 as the predominant ubiquinone and C18: 1v7c as the major fatty acid. The major polar lipids of strain RA1-3^Twere phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain RA1-3^Twas 56 mol% and DNA–DNA relatedness values with the type strains of Octadecabacter temperatus, Octadecabacter antarcticus and Octadecabacter arcticus were 13–24%. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain RA1-3^Tis separated from other recognized species of the genus Octadecabacter. On the basis of the data presented, strain RA1-3^Tis considered to represent a novel species of the genus Octadecabacter, for which the name Octadecabacter ascidiaceicola sp. nov. is proposed. The type strain is RA1-3^T(=5KCTC 42605^T=5CECT 8868^T).