Nonlabens aestuariivivens sp. nov., isolated from a tidal flat

A Gram-stain-negative, non-flagellated, non-gliding, aerobic and rod-shaped bacterium, designated strain OITF-31^T, was isolated from a tidal flat in Oido, an island of South Korea, and subjected to a polyphasic taxonomic study. Strain OITF-31^T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 1.0–2.0% (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain OITF-31^T belonged to the genus Nonlabens, clustering with the type strains of Nonlabens dokdonensis and Nonlabens xylanidelens, sharing 98.1% sequence similarity with each. Strain OITF-31^T exhibited 16S rRNA gene sequence similarities of 93.9–95.8% to the type strains of other Nonlabens species and less than of 91.8% to the type strains of other recognized species. Strain OITF-31^T contained MK-6 as the predominant menaquinone and iso-C_{17: 0} 3-OH and iso-C_{15: 0} as the major fatty acids. The major polar lipids detected in strain OITF-31^T were phosphatidylethanolamine, six unidentified lipids and two unidentified aminolipids. The DNA G+C content of strain OITF-31^T was 38.1 mol% and its mean DNA–DNA relatedness values with the type strains of N. dokdonensis and N. xylanidelens were 24 and 19%, respectively. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain OITF-31^T is separated from recognized species of the genus Nonlabens. On the basis of the data presented, strain OITF-31^T is considered to represent a novel species of the genus Nonlabens, for which the name Nonlabens aestuariivivens sp. nov. is proposed. The type strain is OITF-31^T (=KCTC 52502^T=NBRC 112459^T).