Nocardioides insulae sp. nov., isolated from soil

A Gram-positive, rod-shaped or coccoid bacterial strain, DS-51^T, was isolated from a soil in Dokdo, Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain DS-51^T grew optimally at pH 8.0 and 30°C without NaCl. Phylogenetic analyses based on 16S rRNA qene sequences showed that strain DS-51^T forms a distinct line of descent within the radiation enclosed by the genus Nocardioides. The chemotaxonomic properties of strain DS-51^T were consistent with those of the genus Nocardioides: the cell-wall peptidoglycan type was based on LL-2,6-diaminopimelic acid, MK-8(H₄) was the predominant menaquinone and iso-C_16:0 was the major fatty acid. The DNA G+C content was 71.1 mol%. The 16S rRNA gene sequence of strain DS-51 T had similarity levels of 92.5-95.1 % with the sequences of the type strains of Nocardioides species. Strain DS-51^T could be distinguished from other Nocardioides species by differences in some phenotypic characteristics. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain DS-51^T represents a novel species of the genus Nocardioides, for which the name Nocardioides insulae sp. nov. is proposed. The type strain is DS-51^T (=KCTC 19180^T = DSM 17944^T).