Nocardioides daedukensis sp. nov., a halotolerant bacterium isolated from soil

A Gram-positive, non-motile and rod- or coccoid-shaped bacterial strain, MDN22^T, was isolated from a soil sample from Korea. Strain MDN22 ^T grew optimally at pH 7.0-8.0, at 30 °C and in the presence of 0-0.5% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain MDN22^T was phylogenetically most closely related to the genera Nocardioides and Marmoricola. In the neighbour-joining phylogenetic tree, strain MDN22^T was most closely related to Nocardioides jensenii KCTC 9134^T, with which it exhibited 98.3% 16S rRNA gene sequence similarity. The strain exhibited 93.1-96.9% and 95.3-95.9% 16S rRNA gene sequence similarities to the type strains of other species of the genera Nocardioides and Marmoricola, respectively. The chemotaxonomic properties of strain MDN22^T were consistent with those of the genus Nocardioides; the cell-wall peptidoglycan type was based on LL-2,6-diaminopimelic acid, the predominant menaquinone was MK-8(H4) and the major fatty acids were iso-C _{16 : 0} and C_{17 : 1}. The DNA G+C content was 68.7 mol%. DNA-DNA relatedness data and differential phenotypic properties suggested that strain MDN22^T could be differentiated from N. jensenii and Nocardioides dubius. On the basis of the data obtained, strain MDN22^T is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioides daedukensis sp. nov., is proposed. The type strain is MDN22^T (5KCTC 19601^T=CCUG 57505^T).